摘要
目的 探讨核桃粕酶解多肽的两步酶解制备工艺,研究其体外抗氧化与抗疲劳活性。方法 利用单因素实验及响应面实验找出最优两步酶解制备桃粕酶解多肽方法,并进行二苯基苦基肼基自由基(2,2-diphenyl-1-picrylhydrazyl radical, DPPH)、羟基自由基、超氧阴离子自由基与总还原力的测定判定其抗氧化能力;采用小鼠游泳力竭实验来评估核桃粕酶解多肽的抗疲劳效果。结果 得到制备核桃粕酶解多肽的两部酶解法最优组合为:碱性蛋白酶2000 U/g,碱性蛋白酶pH为9.0,风味蛋白酶用量1000 U/g。与抗坏血酸进行对比得到DPPH自由基清除能力的半抑制浓度为(0.25±0.01) mg/mL、羟基自由基清除能力的半抑制浓度为(0.96±0.01) mg/mL、超氧阴离子自由基清除能力的半抑制浓度为(0.25±0.01) mg/mL,说明核桃粕酶解多肽具有一定的体外抗氧化活性。经过小鼠实验发现,核桃粕酶解多肽对小鼠无负面作用,且可有效延长小鼠游泳时间,维持体内糖原及抗氧化酶含量,降低血清代谢废物及过氧化物的积累,起到一定的抗疲劳效果。结论 通过两步酶解法制备出具有体外抗氧化体内抗疲劳的核桃粕酶解多肽,其在开发具有抗氧化抗疲劳功能的健康食品或功能性食品配料方面具有巨大的应用潜力。
Objective To explore the two-step enzymatic hydrolysis process for preparing walnut meal enzymatic peptides,and study their in vitro antioxidant and anti-fatigue activities.Methods Single-factor experiments and response surface experiments were used to determine the optimal two-step enzymatic hydrolysis method for preparing walnut meal enzymatic peptides.The antioxidant capacity was evaluated by determining the scavenging effects on 2,2-diphenyl-1-picrylhydrazyl(DPPH)radicals,hydroxyl radicals,and superoxide anion radicals,as well as the total reducing power.The anti-fatigue effect was assessed using a mouse swimming exhaustion test.Results The optimal combination for preparing walnut meal enzymatic peptides through two-step enzymatic hydrolysis was as follows:Alkaline protease at 2000 U/g,pH of 9.0 for alkaline protease,and flavor protease at 1000 U/g.Compared with ascorbic acid,the half-maximal inhibitory concentration for DPPH radical scavenging was(0.25±0.01)mg/mL,the half-maximal inhibitory concentration for hydroxyl radical scavenging was(0.96±0.01)mg/mL,and the half-maximal inhibitory concentration for superoxide anion radicals scavenging was(0.25±0.01)mg/mL,indicating that walnut meal enzymatic peptides had certain in vitro antioxidant activity.The mouse experiments revealed that walnut meal enzymatic peptides had no negative effects on mice and could effectively prolong their swimming time,maintain the levels of glycogen and antioxidant enzymes in the body,and reduce the accumulation of serum metabolic waste and peroxides,demonstrating a certain anti-fatigue effect.Conclusion The two-step enzymatic hydrolysis method is successfully used to prepare walnut meal enzymatic peptides with in vitro antioxidant and in vivo anti-fatigue activities,which have great application potential in the development of health foods or functional food ingredients with antioxidant and anti-fatigue functions.
作者
潘静怡
杨悦
王方
PAN Jing-Yi;YANG Yue;WANG Fang(Shaoxing Vocational&Technical College,Shaoxing 312000,China;Luohe Institute of Technology,Henan University of Technology,Luohe 462000,China)
出处
《食品安全质量检测学报》
2026年第1期305-315,共11页
Journal of Food Safety and Quality
关键词
核桃粕
复合酶解
多肽
抗氧化活性
抗疲劳活性
walnut meal
complex enzymatic hydrolysis
polypeptide
antioxidant activity
anti-fatigue activity
