摘要
目的 制备单核细胞增生李斯特氏菌质控样品。方法 根据生长曲线选择进入稳定期的单核细胞增生李斯特氏菌,进行单因素实验和响应面实验,优化冻干保护剂配方;通过均匀性、稳定性等指标对制备出的质控样品进行评价。结果 选择培养12 h进入稳定期的单核细胞增生李斯特氏菌进行冻干保存,优化后的冻干保护剂配方为脱脂乳粉13%、海藻糖8%,谷氨酸钠3%,菌株存活率可达82.27%;在均匀性实验中样品F为2.747,小于F临界值[F_(0.05)(9,10)=3.02];模拟稳定性实验中,运输条件25℃和37℃保存7 d,保存条件4℃和–20℃保存28 d,质控样品稳定性良好。结论 制备的单核细胞增生李斯特氏菌质控菌株可以作为实验室质控样品使用。
Objective To prepare quality control samples of Listeria monocytogenes.Methods Listeria monocytogenes in the stationary phase was selected based on the growth curve.Then single-factor experiments and response surface methodology were used to optimize the lyoprotectant formulation.The prepared quality control samples were evaluated by homogeneity and stability.Results Listeria monocytogenes cultured for 12 hours(stationary phase)was selected for freeze-drying.The optimized lyoprotectant formulation consisted of 13%skim milk powder,8%trehalose and 3%sodium glutamate,achieving a bacterial survival rate of 82.27%.In the homogeneity test,the F-value was 2.747,which was lower than the critical F-value[F_(0.05)(9,10)=3.02].In simulated stability tests,the quality control samples remained stable under transportation conditions which was 25℃and 37℃for 7 days and storage conditions which was 4℃and–20℃for 28 days.The stability of the quality control samples was good.Conclusion The prepared Listeria monocytogenes control strain can be used as a quality control sample in laboratories.
作者
辛乐
史朝烨
王小贺
林芳
束慧丽
XIN Le;SHI Zhao-Ye;WANG Xiao-He;LIN Fang;SHU Hui-Li(Shaanxi Institute for Food and Drug,Xi’an 710000,China;National Medical Products Administration Key Laboratory for Testing Technology of Pharmaceutical Microbiology,Xi’an 710000,China;Shaanxi Key Laboratory of Food and Drug Safety Monitoring,Xi’an 710000,China)
出处
《食品安全质量检测学报》
2026年第1期115-121,共7页
Journal of Food Safety and Quality
基金
陕西省科技厅科技计划项目(2025SF-YBXM-150)。
