摘要
为探究新型糖苷化合物α-水杨苷、α-异水杨苷对罗勒(Ocimum basilicum L.)生长及抗旱性的调控作用,设置α-水杨苷、α-异水杨苷5个浓度(0.005、0.010、0.050、0.100、0.500 mmol/L),并以12%PEG-6000模拟干旱胁迫,系统分析α-水杨苷和α-异水杨苷处理对罗勒种子萌发和幼苗生长的影响。结果显示:在正常生长条件下,0.100 mmol/L的α-水杨苷和α-异水杨苷促进种子萌发和幼苗生长,其中α-水杨苷使发芽率和根长较对照分别提高7.0%和23.8%,α-异水杨苷处理使根长提高37.8%,显著高于α-水杨苷(P<0.05);α-水杨苷和α-异水杨苷处理对可溶性糖含量分别提高45.9%和46.2%,可溶性蛋白含量分别提高33.8%和42.2%,其中α-异水杨苷处理使可溶性蛋白含量的提高幅度高于α-水杨苷。在干旱胁迫下,α-水杨苷和α-异水杨苷处理均能上调超氧化物歧化酶、过氧化氢酶、过氧化物酶活性,降低丙二醛含量,并促进可溶性糖和可溶性蛋白积累,其中α-异水杨苷处理丙二醛的含量低于α-水杨苷处理,可溶性蛋白含量则相反。研究发现,两种化合物作用效果相似,调控作用具有浓度效应,在0.005~0.100 mmol/L范围内,随着浓度升高,促进效果增强;在0.500 mmol/L浓度下部分指标升高变缓或下降。研究首次阐明α构型糖苷通过非水解途径激活抗氧化系统及渗透调节通路。研究结果为基于糖苷构型设计新型植物抗逆剂提供理论支撑。
To investigate the regulatory effects of novel glycoside compounds α-salicin and α-isosalicin on the growth and drought resistance of basil(Ocimum basilicum L.),five concentrations(0.005,0.010,0.050,0.100 and 0.500 mmol/L)of each compound were tested.The effects of α-salicin and α-isosalicin on basil seed germination and seedling growth were systematically evaluated under drought conditions simulated with 12%PEG-6000.The results showed that under normal growth conditions,α-salicin and α-isosalicin(0.100 mmol/L)treatment promoted seed germination and seedling growth.Compared with the control,α-salicin increased the germination rate and root length by 7.0%and 23.8%,respectively.Notably,α-isosalicin treatment at the same concentration led to a 37.8%increase in root length,which was significantly greater than that observed with α-salicin(P<0.05).α-Salicin and α-isosalicin treatment led to increases in soluble sugar content by 45.9%and 46.2%,respectively,and in soluble protein content by 33.8%and 42.2%,respectively.The increase in soluble protein content was significantly greater under α-isosalicin treatment than under α-salicin.When subjected to drought stress,both α-salicin and α-isosalicin treatments markedly upregulated the activity of antioxidant enzymes including superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD),while reducing malondialdehyde(MDA)content.Additionally,these compounds promoted the increase of soluble sugars and proteins.Importantly,α-isosalicin treatment led to a greater reduction in MDA content relative to α-salicin,accompanied by comparatively elevated soluble protein content.This study revealed that the two compounds exhibited similar concentration-dependent regulatory effects.Within the concentration range of 0.005-0.100 mmol/L,their promotive effects increased with rising concentrations;however,at 0.500 mmol/L,the enhancement of certain indicators slowed down or declined.For the first time,this study elucidated that α configured glycosides activate the antioxidant system and osmotic regulation pathway through a non-hydrolytic process.The results of this study provide a theoretical support for designing novel plant antistress agents based on glycoside configuration.
作者
程雨心
孙晶晶
王伟
江承程
王致鹏
郝建华
CHENG Yuxin;SUN Jingjing;WANG Wei;JIANG Chengcheng;WANG Zhipeng;HAO Jianhua(College of Fisheries,Zhejiang Ocean University,Zhoushan 316022,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)
出处
《青岛农业大学学报(自然科学版)》
2025年第4期250-258,共9页
Journal of Qingdao Agricultural University(Natural Science)
基金
国家重点研发计划项目(2022YFC2805101)
青岛海洋科学与技术国家实验室山东省专项经费(2022QNLM0300032)
中国水产科学研究院基本科研业务费资助项目(2023TD71)。
