摘要
探究不同产地、不同生长年限黄芪药材中活性成分的积累分布规律,为有效控制黄芪药材质量提供依据.采用HPLC-ELSD法测定黄芪甲苷含量;HPLC-UVD法测定毛蕊异黄酮葡萄糖苷含量;紫外分光光度法测定总皂苷及总黄酮含量.考察不同产地三年生的黄芪药材,陕西产的黄芪甲苷含量最高,其次是内蒙古、山西、甘肃;毛蕊异黄酮葡萄糖苷含量从高到低依次是甘肃、内蒙古、陕西、山西.考察不同生长年限的陕西黄芪,黄芪甲苷含量随生长年限增加先升后降,3年生黄芪中黄芪甲苷(0.162%)与总皂苷(4.28%)含量最高,随后逐渐下降;毛蕊异黄酮葡萄糖苷及总黄酮含量随生长年限增加逐渐递增,7年生黄芪中毛蕊异黄酮葡萄糖苷(0.0452%)及总黄酮(0.584%)含量最高.若以黄芪甲苷或总皂苷为功效指标,建议选择3年生黄芪;若以毛蕊异黄酮葡萄糖苷或总黄酮为主要功效指标,建议优选6~7年生黄芪药材.
Explore the accumulation and distribution patterns of active ingredients in Astragalus membranaceus from different origins and growth years,providing a basis for effectively controlling the quality of Astragalus membranaceus.The content of astragaloside IV was determined using HPLC-ELSD,while Calycosin-7-O-β-D-glucoside was determined using HPLC-UVD.Total saponins and total flavonoids were quantified by ultraviolet spectrophotometry.Among three-year-old Astragalus samples from different origins,Shaanxi exhibited the highest astragaloside IV content,followed by Inner Mongolia,Shanxi,and Gansu.The Calycosin-7-O-β-D-glucoside content ranked from highest to lowest as Gansu,Inner Mongolia,Shaanxi,and Shanxi.For Shaanxi Astragalus of different growth years,three-year-old samples showed the highest astragaloside IV(0.162%)and total saponin(4.28%)contents,which subsequently declined.The Calycosin-7-O-β-D-glucoside(0.0452%)and total flavonoid(0.584%)contents peaked in seven-year-old samples.For medicinal use targeting astragaloside IV or total saponins,harvesting three-year-old Astragalus is optimal;for Calycosin-7-O-β-D-glucoside or total flavonoids,six to seven-year-old Astragalus is preferred.
作者
李楠
李莎莎
苏格萍
郭佳琪
周恬甜
屈雯
LI Nan;LI Sha-sha;SU Ge-ping;GUO Jia-qi;ZHOU Tian-tian;QU Wen(School of Food Science and Engineering,Xi′an Key Laboratory of Precision Nutrition and Functional Products,Shaanxi University of Science&Technology,Xi′an 710021,China;Shaanxi Sun Simiao Traditional Chinese Medicine Co.,Ltd.,Xi′an 710299,China)
出处
《陕西科技大学学报》
北大核心
2026年第1期69-76,共8页
Journal of Shaanxi University of Science & Technology
基金
陕西省重点研发-重点产业创新链(群)-农业领域科技计划项目(2024NC-ZDCYL-04-14)
陕西省科技厅重点研发-关键核心技术攻关-社会发展领域科技计划项目(2024SF-GJHX-08)
陕西省西安市未央区科技计划项目(202309)
桂林医学院广西药物分子发现与成药性优化重点实验室开放基金项目(GKLDDO-2024-03)。
关键词
黄芪
产地
生长年限
黄芪甲苷
毛蕊异黄酮葡萄糖苷
质量标志物
Astragalus membranaceus
origins
growth years
astragaloside IV
calycosin-7-O-β-D-glucoside
quality markers
