摘要
在过去十年中,因全球严重的雾霾污染,学术界对大气细颗粒物(PM_(2.5))的毒性及其致毒机制展开了系统性研究。细胞实验具有操作简便、耗时短及重复性强等优点,已成为目前研究PM_(2.5)毒性的主要方法。其中,人肺泡上皮A549细胞由于相对稳定的生物学特性及持续增殖的能力,在PM_(2.5)毒性检测实验中得到广泛应用。该文整理综述了PM_(2.5)对A549细胞的综合毒性及其不同组分的细胞毒性,梳理总结了染毒前PM_(2.5)的分离及不同组分的提取方法,并对现有的细胞毒性测定方法进行了整理与比较,旨在为大气颗粒物及其他颗粒物的细胞毒性研究提供参考,有助于深入探究颗粒物的毒理机制,并优化相关领域的检测技术。
Over the past decade,the global prevalence of severe haze pollution has prompted the academic community to engage in systematic investigations into the toxicity of atmospheric fine particulate matter(PM_(2.5))and its underlying toxicological mechanisms.In this context,cellular experiments have emerged as a predominant methodology for assessing PM_(2.5) toxicity,owing to their operational simplicity,efficiency,and high reproducibility.Notably,human lung epithelial A549 cells are frequently employed in toxicity testing due to their relatively stable biological properties and capacity for continuous proliferation.This article reviews the comprehensive toxicity of PM_(2.5) on A549 cell and the cytotoxicity of its different components,summarizes the methods for isolating PM_(2.5) and extracting its various components before exposure,and organizes and compares existing cytotoxicity measurement methods.The aim is to provide a reference for the study of the cytotoxicity of atmospheric particles and other particulate matter,which will help to further explore the toxicological mechanisms of particulate matter and optimize detection technologies in related fields.
作者
解姣姣
付依然
陈鹏宇
马雅文
苑春刚
XIE Jiao-jiao;FU Yi-ran;CHEN Peng-yu;MA Ya-wen;YUAN Chun-gang(Department of Environmental Science and Engineering,North China Electric Power University,Baoding 071000,China)
出处
《分析测试学报》
北大核心
2026年第1期209-216,共8页
Journal of Instrumental Analysis
基金
国家自然科学基金项目(22206049)
中央高校基本科研业务费专项资金(2024MS174)。
