摘要
Aux/IAA基因家族成员编码的蛋白通过调节生长素(IAA)信号转导途径调控植物的胚胎形成、种子发育等多种发育过程。为探究Aux/IAA基因家族在枣胚发育过程中的作用,利用胚高度可育枣品种灰枣和胚低度可育枣品种孔府酥脆枣的转录组数据,对影响枣胚发育过程的Aux/IAA基因家族的关键基因进行生物信息学分析和功能验证。结果表明,经过基因克隆获得了一个长度为1731 bp的Aux/IAA基因家族的基因序列,编码362个氨基酸。蛋白质结构预测显示,其二级结构以无规则卷曲为主,且具有典型的IAA9蛋白保守域,将其命名为ZjIAA9。同源性分析结果显示,ZjIAA9与红栎中的RGQ29_009000、栓皮栎中的CFP56_014209亲缘关系较近。通过农杆菌介导的叶盘法转基因获得了Micro-Tom小番茄转基因植株。对Micro-Tom小番茄转基因植株的生长素含量进行测定后发现,转基因植株果肉的IAA含量高于未转基因植株,表明ZjIAA9可能参与生长素的生物合成。与野生型相比,转基因植株的表现为无籽或少籽状,表明枣ZjIAA9可能参与调控枣胚胎发育过程。
The members of the Aux/IAA gene family encode proteins that regulate various developmental processes in plants,such as embryogenesis and seed development,by modulating the auxin(IAA)signal transduction pathway.To explore the role of Aux/IAA family genes in jujube embryo development,this study performed bioinformatics analysis and functional verification on the key genes of the Aux/IAA family that affect jujube embryo development,using transcriptome data of Huizao(a jujube variety with high embryo fertility)and Kongfusucui(a jujube variety with low embryo fertility).Results showed that an Aux/IAA family gene sequence with a length of 1731 bp was obtained via gene cloning,which encoded 362 amino acids.Protein structure prediction indicated that its secondary structure was mainly composed of random coil and that it had a typical conserved domain of the IAA9 protein;thus,it was named ZjIAA9.Homology analysis revealed that ZjIAA9 was closely related to RGQ29_009000 in Quercus rubra and CFP56_014209 in Quercus suber.Transgenic Micro-Tom tomato plants were obtained via Agrobacterium-mediated leaf disc transformation.Determination of auxin content in transgenic Micro-Tom tomato plants showed that the IAA content in the pulp of transgenic plants was higher than that in non-transgenic plants,indicating that ZjIAA9 may regulate auxin biosynthesis.Compared with wild-type plants,transgenic plants showed a seedless or few-seeded phenotype,suggesting that jujube ZjIAA9 may be involved in regulating jujube embryo development.
作者
杨梦帆
马晴
任海燕
王永康
赵爱玲
薛晓芳
苏万龙
石美娟
刘丽
李毅
YANG Mengfan;MA Qing;REN Haiyan;WANG Yongkang;ZHAO Ailing;XUE Xiaofang;SU Wanlong;SHI Meijuan;LIU Li;LI Yi(College of Horticulture,Shanxi Agricultural University,Jinzhong 030801,China;Pomology Institute,Shanxi Agricultural University,Shanxi Key Laboratory of Fruit Germplasm Creation and Utilization,Taiyuan 030031,China)
出处
《华北农学报》
北大核心
2025年第6期77-84,共8页
Acta Agriculturae Boreali-Sinica
基金
山西省科技重大专项计划“揭榜挂帅”项目(202201140601027)
山西省自然科学基金项目(202103021224147)
农业农村部种质资源保护专项(111721301354052002)
国家科技资源共享服务平台项目(NHGRC2023-NH12-1)
现代农业产业技术体系专项(CARS-30-1-07,CARS-30-ZZ-22)。
关键词
枣
胚发育
ZjIAA9
生物信息学分析
功能验证
Jujube
Embryo development
ZjIAA9
Bioinformatics analysis
Functional verification
