摘要
富含半胱氨酸类受体激酶作为受体激酶重要亚家族,在植物生长发育中发挥关键作用。旨在解析GhCRK26基因与棉花纤维发育的关系,为棉花纤维品质改良提供理论基础。选用陆地棉系9和海岛棉新海16,采集纤维发育不同时期样本及根、茎、叶组织。通过qRT-PCR分析GhCRK26基因表达模式;利用PCR技术克隆GhCRK26基因;借助生物信息学工具构建系统发育树,预测蛋白理化性质、跨膜结构及信号肽;运用PlantCare数据库分析启动子顺式作用元件;通过亚细胞定位试验明确蛋白定位。结果表明,GhCRK26基因在2个品种开花后10,20,30 d的表达量呈现极显著差异;在系9中叶片的表达水平最高,在新海16中,茎与叶的表达量未表现出显著差异。成功克隆获得2049 bp的CDS序列,编码682个氨基酸。进化树显示,GhCRK26蛋白与野西瓜苗亲缘关系最远,与夏威夷棉、海岛棉最近;该蛋白为亲水不稳定蛋白,含跨膜结构和信号肽;启动子区鉴定到茉莉酸甲酯和脱落酸响应元件;亚细胞定位证实其定位于细胞膜。以上研究为进一步深入解析GhCRK26基因在纤维发育中的功能提供了依据。
As an important subfamily of receptor kinases,cysteine-rich receptor kinases play a key role in plant growth and development.The aim of this study was to analyze the relationship between the GhCRK26 gene and the development of cotton fiber,and to provide a theoretical basis for the improvement of cotton fiber quality.This study selected upland cotton Line 9 and sea island cotton Xinhai 16,and collected samples at different periods of fiber development and root,stem and leaf tissues.The expression pattern of GhCRK26 was analyzed by qRT-PCR;the gene was cloned by PCR;a phylogenetic tree was constructed with the help of bioinformatics tools to predict the physicochemical properties of the protein,its transmembrane structure and signal peptide;the promoter cis-acting elements were analyzed by the PlantCare database;and the localization of the protein was clarified by the subcellular localization assay.The results showed that the expression of GhCRK26 gene showed highly significant differences at 10,20 and 30 days after flowering in two varieties;the highest expression levels were found in the leaves of Line 9,while in Xinhai 16,the expression of stem and leaves did not show significant differences.A 2049 bp CDS sequence encoding 682 amino acids was successfully cloned.The evolutionary tree showed that GhCRK26 protein was the most distantly related to Hibiscus trionum,and the closest to Gossypium tomentosum and Gossypium barbadense;the protein was hydrophilic and unstable,containing a transmembrane structure and a signal peptide;methyl jasmonate and abscisic acid response elements were identified in the promoter region;and the subcellular localization confirmed that it was localized in the cell membrane.The above studies provide a basis for further in-depth analysis of the function of GhCRK26 gene in fiber development.
作者
李静
庞博
张茹
扎尔加玛丽·阿不都别克
王正瑞
史顺宇
范珍珍
马晶晶
陈佳林
宋武
李生梅
高文伟
LI Jing;PANG Bo;ZHANG Ru;ZHAERJIAMALI Abudubieke;WANG Zhengrui;SHI Shunyu;FAN Zhenzhen;MA Jingjing;CHEN Jialin;SONG Wu;LI Shengmei;GAO Wenwei(Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Agricultural Vocational and Technical University,Changji 831100,China)
出处
《华北农学报》
北大核心
2025年第6期62-70,共9页
Acta Agriculturae Boreali-Sinica
基金
国家重点研发计划子课题(2021YFD1900802-4)
新疆维吾尔自治区“天池英才”引进计划项目
新疆维吾尔自治区自然科学基金项目(2025D01B96)
2024年度新疆农业职业技术大学资助课题(XJNZYBS202401)。
