摘要
三七花的品质显著受到栽培模式的影响,而甲羟戊酸途径在三萜皂苷生物合成途径中扮演着关键角色。然而,关于三七花甲羟戊酸途径关键基因表达情况尚不清楚。本研究采集了三七-华山松和三七-云南松系统中的三七花,基于前期转录组数据,设计引物并利用RT-PCR克隆出甲羟戊酸二磷酸脱羧酶(MVD酶)基因,将其命名为PnMVD1。结果显示:PnMVD1基因的CDNA全长为1263 bp,编码一条由421个氨基酸组成的多肽链,其分子量为46.53641 kD。同时,PnMVD1基因属于GHMP激酶蛋白家族,拥有GHMP激酶超家族特征性识别序列,并且与越南参MVD基因显示出极高的同源性(达到98.81%)。荧光定量PCR表明,三七花PnMVD1基因的表达量在三七-华山松系统中显著高于三七-云南松系统。两种栽培模式下,三七花PnMVD1基因的表达水平在初花期高于盛花期。此外,三七花PnMVD1基因的表达水平与温度和压强呈显著正相关,而与湿度和海拔呈显著负相关。本研究为深入理解三七花PnMVD1基因在促进皂苷生物合成过程中的关键作用奠定了基础,不仅为揭示三七花甲羟戊酸途径关键基因的表达机制提供了重要线索,也为通过调控环境因子来优化三七花的栽培模式、提高三七花的品质提供了理论依据。
The quality of Panax notoginseng flowers is significantly influenced by cultivation patterns,and the mevalonate pathway plays a crucial role in the biosynthesis of triterpenoid saponins.However,the expression of key genes in the mevalonate pathway of P.notoginseng flowers remains unclear.In this study,P.notoginseng flowers from the P.notoginseng-Pinus armandii and P.notoginseng-Pinus yunnanensis systems were collected.Based on preliminary transcriptome data,primers were designed,and the mevalonate diphosphate decarboxylase(MVD)gene was cloned using RT-PCR,which was named PnMVD1.The results showed that the full-length cDNA of the PnMVD1 gene is 1263 bp,encoding a polypeptide chain consisting of 421 amino acids with a molecular weight of 46.53641 kD.Additionally,the PnMVD1 gene belongs to the GHMP kinase protein family,possessing the characteristic recognition sequence of the GHMP kinase superfamily,and exhibits extremely high homology(98.81%)with the MVD gene of Panax vietnamensis.Quantitative real-time PCR revealed that the expression level of the PnMVD1 gene in P.notoginseng flowers from the P.notoginseng-Pinus armandii system was significantly higher than that from the P.notoginseng-Pinus yunnanensis system.Under both cultivation patterns,the expression level of the PnMVD1 gene in P.notoginseng flowers was higher during the initial flowering stage than during the full flowering stage.Furthermore,the expression level of the PnMVD1 gene showed a significant positive correlation with temperature and atmospheric pressure,while exhibiting a significant negative correlation with relative humidity and altitude.This study lays a foundation for a deeper understanding of the critical role of the PnMVD1 gene in promoting saponin biosynthesis.It not only provides important clues for elucidating the expression mechanisms of key genes in the mevalonate pathway of P.notoginseng flowers but also offers a theoretical basis for optimizing cultivation patterns and improving the quality of P.notoginseng flowers by regulating environmental factors.
作者
王颜
李玥
王澍
何霞红
芮蕊
WANG Yan;LI Yue;WANG Shu;HE Xiahong;RUI Rui(College of Landscape Architecture and Horticulture,Southwest Forestry University,Kunming Yunnan 650233,P.R.China;Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource,Southwest Forestry University,Kunming Yunnan 650233,P.R.China)
出处
《西部林业科学》
北大核心
2025年第6期56-63,共8页
Journal of West China Forestry Science
基金
云南基础研究项目(202501BD070001-087,202401BD070001-122)
云南基础研究重点项目(202501BD070001-023)共同资助。
关键词
三七花
PnMVD1基因
基因克隆
表达
Panax notoginseng flower
PnMVD1 gene
gene cloning
gene expression
