摘要
目的观察断食对D-半乳糖诱导的衰老小鼠皮肤老化的影响。方法选取18只SPF级雄性C57BL/6小鼠,随机分为空白组、D-半乳糖模型组和断食组,每组6只。除空白组外,给予其余两组小鼠D-半乳糖腹腔注射以制备衰老小鼠模型。造模同时,给予断食组小鼠断食干预,其余两组小鼠均正常饮食,共干预12周。干预期间,观察各组小鼠的生理及皮肤状态,并定期进行体重检测。干预12周后处死小鼠,应用HE染色和胶原纤维-弹力纤维复合染色试剂盒(EVG-Weigert)观察小鼠皮肤形态学改变,检测小鼠皮肤组织和血清过氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(HYP)含量。结果随着干预持续进行,D-半乳糖模型组小鼠出现明显衰老体征,而断食组小鼠各项生理状态及皮肤老化表现均较D-半乳糖模型组有所改善。D-半乳糖模型组小鼠体重变化情况与空白组基本一致,断食组小鼠体重明显低于其余两组。与空白组比较,D-半乳糖模型组小鼠皮肤的表皮、真皮厚度变薄,弹力纤维面积减少(P<0.05);与D-半乳糖模型组比较,断食组小鼠皮肤的表皮、真皮厚度变厚,弹性纤维面积增加(P<0.05)。与空白组比较,D-半乳糖模型组小鼠皮肤组织和血清SOD、HYP含量降低,血清MDA含量升高(P<0.05);与D-半乳糖模型组比较,断食组小鼠血清SOD、HYP含量升高,皮肤组织和血清MDA含量降低(P<0.05)。结论断食对衰老小鼠模型的皮肤老化具有保护作用,表现为改善表皮和真皮萎缩,以及弹力纤维的断裂和降解,其潜在机制与其对机体整体抗氧化能力的提升有关。
Objective To observe the effect of intermittent fasting on D-galactose-induced skin aging in aging mice.Methods Eighteen SPF-grade male C57BL/6 mice were selected and randomly divided into blank group,D-galactose model group,or intermittent fasting group,with 6 mice in each group.Except for the blank group,the remaining two groups received intraperitoneal injections of D-galactose to establish an aging mouse model.Simultaneously with modeling,the intermittent fasting group was subjected to intermittent fasting intervention,while the remaining two groups received a normal diet.The intervention lasted for 12 weeks.During the intervention,the physiological and skin conditions of the mice in each group were observed,and body weight was measured regularly.After 12 weeks of intervention,the mice were killed.HE staining and collagen fiber and elastic fiber staining kit(EVG-Weigert)were used to observe morphological changes in the mouse skin.The contents of superoxide dismutase(SOD),malondialdehyde(MDA),and hydroxyproline(HYP)in skin tissue and serum were detected.Results As the intervention progressed,the D-galactose model group demonstrated obvious signs of aging,while the intermittent fasting group exhibited improvements in various physiological states and skin aging manifestations compared to the D-galactose model group.Body weight changes in the D-galactose model group were largely consistent with the blank group,while the intermittent fasting group exhibited significantly lower body weight than the remaining two groups.Compared with the blank group,the epidermis and dermis thickness were thinner,and the elastic fiber area was reduced in the skin of the D-galactose model group(P<0.05).Compared with the D-galactose model group,the intermittent fasting group showed thicker epidermis and dermis and an increased elastic fiber area(P<0.05).Compared with the blank group,the contents of SOD and HYP in both skin tissue and serum were reduced,while serum MDA content elevated in the D-galactose model group(P<0.05).Compared with the D-galactose model group,the intermittent fasting group yielded elevated serum SOD and HYP contents,whereas lower MDA contents in both skin tissue and serum(P<0.05).Conclusion Intermittent fasting may have a protective effect against skin aging in aging mouse model,manifested by improvements in epidermal and dermal atrophy,as well as the fragmentation and degradation of elastic fibers.The potential mechanism is related to its enhancement of the body′s overall antioxidant capacity.
作者
郭楷扬
颜萍
叶尔扎提·叶尔肯
祝安然
罗明洁
GUO Kaiyang;YAN Ping;Yeerzhati Yeerken;ZHU Anran;LUO Mingjie(School of Nursing,Xinjiang Medical University,Urumqi 830001,Xinjiang,China;Xinjiang Regional Research Center for Population Diseases and Health Care,Urumqi 830001,Xinjiang,China)
出处
《广西医学》
2025年第12期1776-1782,共7页
Guangxi Medical Journal
基金
新疆维吾尔自治区科技支疆项目(2022E02119)
博士科研启动金(0103010211)。
关键词
皮肤老化
断食
D-半乳糖
小鼠
Skin aging
Intermittent fasting
D-galactose
Mice
