摘要
目的 采用超高效液相色谱-四极杆-飞行时间质谱(UHPLC-Q-TOF/MS)技术鉴别肺宁1号方入血成分,为后续该方药效物质基础及作用机制研究提供依据。方法 将20只SD大鼠随机分成2组,每组各10只,分别灌胃给予20 g/kg肺宁1号方汤剂及生理盐水,连续灌胃7 d后收集两组大鼠血清。基于中医药整合药理学研究平台v2.0(TCMIP)、TCMID、TCM@taiwan、中医百科平台、上海中科院化学专业数据库、Pub Chem、中国知网、Chemical Book 、MS-DIAL、化源网等数据库以及查询《中华人民共和国药典》、《中华本草》等文献资料建立肺宁1号方化学成分列表,并结合UHPLC-Q-TOF/MS仪器自带谱库和对照品分别靶向定性检测两组大鼠的血清以及肺宁1号方化学成分,最后根据色谱峰保留时间、质荷比偏差、同位素分布以及质谱碎裂规律筛选鉴别出含药血清中的入血中药成分。结果在正、负离子模式下共鉴定出49种入血成分,主要为生物碱类、黄酮类、苯丙素类、甾体类、挥发油类、有机酸类以及各种萜类成分。结论 本研究初步建立了高效鉴别肺宁1号方入血原型成分的方法,为后续阐明该方药效物质基础及作用机制提供一定的研究基础。
Objective To identify the components absorbed into serum of Feining No.1 Recipe using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UHPLC-Q-TOF/MS)technology,providing a basis for subsequent research on the pharmacodynamic material basis and mechanism of action of this recipe.Methods Twenty SD rats were randomly divided into two groups,with 10 rats in each group.They were orally administered Feining No.1 Recipe decoction(20 g/kg)and normal saline respectively,once daily for 7 consecutive days.Serum was collected from both groups after the final administration.Based on databases including the Integrative Pharmacologybased Research Platform of Traditional Chinese Medicine V2.0(TCMIP),TCMID,TCM@taiwan,the Encyclopedia of Traditional Chinese Medicine Platform,the Shanghai Chinese Academy of Sciences Chemical Professional Database,PubChem,CNKI,ChemicalBook,MSDIAL,and ChemSrc,along with consultations of literature such as the Pharmacopoeia of the People’s Republic of China and Chinese Materia Medica,a chemical constituent list for the Feining No.1 Recipe was established.Using UHPLC-Q-TOF/MS with its built-in spectral library and reference standards,targeted qualitative analysis was performed on the serum samples from both groups and the chemical components of Feining No.1 Recipe.Finally,the components absorbed into serum derived from the recipe in the medicated serum were screened and identified based on chromatographic peak retention time,mass-to-charge ratio deviation,isotopic distribution,and mass spectrometry fragmentation patterns.Results A total of 49 components absorbed into serum were identified in both positive and negative ion modes,mainly including alkaloids,flavonoids,phenylpropanoids,steroids,volatile oils,organic acids,and various terpenoids.Conclusion This study preliminarily established an efficient method for identifying the prototype components absorbed into serum of Feining No.1 Recipe,providing a research foundation for further elucidating the pharmacodynamic material basis and mechanism of action of this recipe.
作者
王珠强
龙永树
彭伟文
康雨欣
黄莹
黄振炎
WANG Zhuqiang;LONG Yongshu;PENG Weiwen(Pharmacology Laboratory,Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine,Zhongshan 528401,China;不详)
出处
《中国处方药》
2025年第24期32-36,共5页
Journal of China Prescription Drug
基金
2024年度中山市医学科研项目(20241A020099)
2024年度研究生及规培医师科研创新项目立项(2024KCY11)
2025中医药部门中央补助资金(中医药传承创新发展示范试点项目)制剂提升项目(4420000002811049-2025)。
