摘要
目的探讨益气活血方对乙型肝炎小鼠的改善作用及对STAT3通路的影响。方法将C57BL/6小鼠随机分为空白对照组、模型组、益气活血方低剂量组(10 g/kg)、高剂量组(20 g/kg)。除空白对照组外,其余各组小鼠通过尾静脉注射重组腺病毒载体AAV8-1.3HBV构建乙型肝炎小鼠模型。检测小鼠血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、γ-谷氨酰转肽酶(γ-GT)及总胆红素(TBIL)水平;ELISA检测小鼠血清乙型肝炎e抗原(HBeAg)和乙型肝炎表面抗原(HbsAg)水平;检测血浆凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fib)水平;HE染色检测小鼠肝组织病理变化;Masson染色检测肝组织纤维化情况;Western blot检测肝组织中信号转导及转录激活因子3(STAT3)与磷酸化STAT3(p-STAT3)蛋白的表达。结果益气活血方显著降低小鼠血清AST、ALT、γ-GT及TBIL水平;显著降低小鼠血清HBeAg与HbsAg表达水平;显著缩短PT、APTT、TT,且显著升高Fib水平;明显减轻肝脏组织病理损伤和纤维化程度,显著降低肝组织中p-STAT3/STAT3比值。结论益气活血方通过抑制STAT3活性改善乙型肝炎小鼠肝功能与凝血功能,减轻肝纤维化。
Objective To explore the improvement of Yiqi Huoxue decoction on hepatitis B mice and its influence on STAT3 pathway.Methods C57BL/6 mice were randomly divided into control group,model group,low-dose Yiqi Huoxue group(10 g/kg),and high-dose group(20 g/kg).Mice in all groups,except for the blank control group,were injected with the recombinant adenovirus vector AAV8-1.3HBV via the tail vein to establish a hepatitis B mouse model.The levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),γ-glutamyl transpeptidase(γ-GT)and total bilirubin(TBIL)in serum of mice were detected.ELISA was used to detect the levels of hepatitis Be antigen(HBeAg)and hepatitis B surface antigen(HbsAg)in serum of mice.The plasma prothrombin time(PT)activated partial prothrombin time(APTT),thrombin time(TT),and fibrinogen(Fib)levels were also assessed.Pathological changes in liver tissue of mice were examined by using HE staining,while Masson staining was employed to evaluate hepatic fibrosis.Western blot was used to detect the expressions of signal transducer and activator of transcription 3(STAT3)and phosphorylated STAT3(p-STAT3)in liver tissues of mice.Results Yiqi Huoxue decoction reduced the levels of AST,ALT,γ-GT and TBIL in mice,reduced the levels of HBeAg and HbsAg,shorten PT,APTT and TT,and increased the level of Fib,alleviated the pathological damage and fibrosis degree of liver tissue,and reduced the p-STAT3/STAT3 ratio.Conclusion Yiqi Huoxue decoction could enhance liver function and coagulation in hepatitis B mice by inhibiting STAT3 activity,alleviating liver fibrosis.
作者
韩莎
安静
唐利
王素青
李会龙
HAN Sha;AN Jing;TANG Li;WANG Su-qing;LI Hui-long(Department of Clinical Laboratory,The Second Affiliated Hospital of Xingtai Medical College,Xingtai 054199,China;Physical Examination Center,The Second Affiliated Hospital of Xingtai Medical College,Xingtai 054199,China;Department of Gastroenterology,The Second Affiliated Hospital of Xingtai Medical College,Xingtai 054199,China)
出处
《解剖科学进展》
2025年第5期675-678,共4页
Progress of Anatomical Sciences
基金
邢台市重点研发计划项目(2023ZC144)。
