摘要
硝化抑制剂可通过抑制氨氧化细菌(ammonia-oxidizing bacteria,AOB)的活性影响土壤中铵态氮转化为硝态氮的生物转化过程。【目的】研究硝化抑制剂3,4-二甲基吡唑磷酸盐(3,4-dimethylpyrazole phosphate,DMPP)对滨海盐碱水稻土中AOB群落结构及其构建机制的影响。【方法】以典型的硝化抑制剂DMPP为材料,基于盆栽试验和高通量测序技术研究在2种盐度条件下添加DMPP对土壤AOB多样性、群落结构和群落组装过程的影响。【结果】添加DMPP提高了土壤AOB群落的α多样性,且在高盐土壤中达到了显著水平;添加DMPP显著改变了AOB的群落组成,降低了高相对丰度类群的相对丰度,富集低相对丰度类群,且高相对丰度类群AOB相对丰度的降低是DMPP抑制硝化作用过程的主要原因;主坐标分析发现,添加DMPP后AOB的群落结构发生明显变化,且高盐土壤中的变化大于低盐土壤;零模型分析结果显示,随机性过程在AOB群落组装过程中起主导作用,且添加DMPP后随机性过程的贡献更大;典范对应分析和曼特尔检验表明,土壤酸碱度、电导率、有机质、总氮和碱解氮是影响AOB群落结构变化的主要理化因子。【结论】添加DMPP对不同盐分含量的滨海盐碱水稻土中AOB群落具有显著影响,且DMPP在不同盐度土壤中的抑制效果不同。
Nitrification inhibitors can affect the biological transformation process of ammonium nitrogen to nitrate nitrogen in soil by inhibiting the activity of ammonia-oxidizing bacteria(AOB).[Objective]To investigate the effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate(DMPP)on the community structure and assembly mechanisms of AOB in coastal saline-alkaline paddy soil.[Methods]To study the effects of the typical nitrification inhibitor DMPP addition on the diversity,community structure,and community assembly process of AOB in soil under two salinity levels.Pot experiments and high-throughput sequencing were employed to determine the diversity,community structure,and community assembly process of AOB.[Results]The addition of DMPP increased the alpha diversity of AOB in soil,which reached a significant level in the highsalinity soil.The addition of DMPP significantly changed the community composition of AOB,reducing the relative abundance of taxa with high relative abundance and enriching the taxa with low relative abundance.The decrease in relative abundance of taxa with high relative abundance was the main reason for the inhibition of DMPP on nitrification.Principal coordinates analysis revealed that the community structure of AOB changed significantly after the addition of DMPP,which was more obvious in high-salinity soil.The null model analysis results showed that stochastic processes played a dominant role in the community assembly process of AOB,and the contribution of stochastic processes increased after the addition of DMPP.Canonical correspondence analysis and Mantel’s test indicated that soil pH,electrical conductivity,organic matter,total nitrogen,and alkaline-hydrolyzable nitrogen were the main physicochemical factors influencing changes in AOB community structure.[Conclusion]DMPP exerted significant impacts on AOB communities in coastal saline-alkaline paddy soils across varying salinity levels,with its inhibitory effects varying substantially with soil salinity.
作者
周家庆
徐敏
汪峰
刘浩
朱诗君
曹慧
ZHOU Jiaqing;XU Min;WANG Feng;LIU Hao;ZHU Shijun;CAO Hui(Key Laboratory of Agricultural Environmental Microbiology,Ministry of Agriculture and Rural Affairs,College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu,China;Ningbo Key Laboratory of Quality and Safety Detection and Control of Characteristic Agricultural Products,Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang,China)
出处
《微生物学报》
北大核心
2025年第12期5617-5629,共13页
Acta Microbiologica Sinica
基金
国家自然科学基金(32471725)
宁波市“科技创新2025”重大专项(2022Z169,2023Z114)。
关键词
3
4-二甲基吡唑磷酸盐
氨氧化细菌
群落多样性
群落组成
群落组装
3,4-dimethylpyrazole phosphate(DMPP)
ammonia-oxidizing bacteria(AOB)
community diversity
community composition
community assembly
