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酵母膳食纤维通过调节酵母抗氧化系统缓解砷引起的细胞凋亡

Yeast dietary fiber regulates arsenic-induced apoptosis in yeast cells by modulating the antioxidant system
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摘要 【目的】以模式生物酿酒酵母为材料,研究酵母膳食纤维(yeast dietary fiber, YDF)在砷诱导酵母细胞凋亡过程中的缓解作用。【方法】运用平板涂布法、分光光度法、荧光显微技术以及逆转录实时定量聚合酶链式反应(quantitative reverse transcription polymerase chain reaction, RT-qPCR)法检测酵母细胞的相对存活率、细胞凋亡及抗氧化相关指标。【结果】砷可显著降低酵母细胞的相对存活率,提高胞内活性氧(reactive oxygen species, ROS)水平和丙二醛(malondialdehyde, MDA)含量,诱导细胞发生凋亡。当YDF(0.5 mg/mL或1.0 mg/mL)与砷共同作用时,酵母细胞的相对存活率、抗氧化分子谷胱甘肽(glutathione, GSH)含量、抗氧化酶[超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase, GPX)]活性以及抗氧化酶基因(SOD1、GPX2、CTA1和CTT1)的相对表达量均高于砷单独处理组;同时,ROS水平和MDA含量下降,促凋亡基因(AIF1、NMAⅢ和NUC1)的相对表达量显著下调,发生凋亡的细胞数量显著减少。【结论】YDF能够通过调节抗氧化系统降低砷胁迫引发的细胞毒性,进而缓解砷诱导的细胞凋亡。 [Objective]To investigate the effect of yeast dietary fiber(YDF)on arsenic-induced apoptosis in Saccharomyces cerevisiae and decipher the possible mechanism.[Methods]The relative survival rate,apoptosis,and antioxidant indicators were determined by the spread plate method,spectrophotometry,fluorescence microscopy,and RT-qPCR.[Results]The exposure to arsenic significantly decreased the relative survival rate,elevated the intracellular reactive oxygen species(ROS)and malondialdehyde(MDA)levels,and induced apoptosis.However,in the presence of YDF(0.5 mg/mL or 1.0 mg/mL)and arsenic,the arsenic-induced toxic effects were effectively attenuated,which was evidenced by increases in the relative survival rate,content of glutathione,activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GPX),and relative expression of antioxidant enzyme genes(SOD1,CTA1,CTT1,and GPX2).Moreover,the treatment with both YDF and arsenic lowered the ROS and the MDA levels,significantly down-regulated the relative expression levels of pro-apoptotic genes(AIF1,NMAⅢ,and NUC1),and significantly reduced apoptotic cells compared with the treatment with arsenic alone.[Conclusion]YDF regulates the antioxidant system to attenuate the arsenic-induced cytotoxicity,thereby alleviating the arsenic-induced apoptosis.
作者 吴丽华 葛文君 姚夏 董娓伶 赵文婧 WU Lihua;GE Wenjun;YAO Xia;DONG Weiling;ZHAO Wenjing(College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong,Shanxi,China)
机构地区 太原师范学院生物科学与技术学院
出处 《微生物学报》 北大核心 2025年第12期5352-5361,共10页 Acta Microbiologica Sinica
基金 国家自然科学基金(21307087) 山西省高校科技创新项目(2019L0820) 山西省大学生创新项目(20251297)。
关键词 酵母膳食纤维 酿酒酵母 亚砷酸钠 凋亡 抗氧化系统 yeast dietary fiber Saccharomyces cerevisiae sodium arsenite apoptosis antioxidant system
分类号 R151 [医药卫生—营养与食品卫生学]
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