摘要
β-桶组装机器(β-barrel assembly machinery, BAM)复合物是革兰氏阴性菌中负责β-桶状外膜蛋白(outer membrane proteins, OMPs)在外膜中组装的重要装置,其功能缺陷可导致细菌死亡,因而成为抗菌药物研发的新型靶点。不同来源细菌中BAM复合物的亚基组成存在差异,大肠杆菌BAM复合物由核心亚基BamA及辅助脂蛋白BamB-E组成,其中BamA作为Omp85家族成员通过其β-桶结构的动态构象变化,并在脂蛋白的辅助下介导底物OMPs的折叠与释放。本文综述了大肠杆菌中BAM复合物最小功能单元、完整功能单元以及其他功能单元的研究进展,并总结了靶向BAM复合物的相关药物筛选情况,为克服革兰氏阴性菌耐药性提供了新策略。
The β-barrel assembly machinery(BAM)complex is an essential apparatus that is responsible for the assembly of β-barrel outer membrane proteins(OMPs)into the outer membrane of Gram-negative bacteria.Its functional defects can lead to bacterial death,and thus it is established as a new target for antibacterial drug development.The subunit composition of the BAM complex varies across different bacterial species and in Escherichia coli,it is composed of a core subunit BamA and auxiliary lipoproteins BamB−E.BamA,as a member of the Omp85 family,mediates the folding and release of substrate OMPs through the dynamic conformational changes of its β-barrel structure that are regulated by lipoproteins.In the present review,we summarized recent progress in distinguishing the minimal functional unit,complete functional unit,and other functional units of the BAM complex in E.coli.Moreover,by reviewing the drug screening studies targeting the BAM complex,we provided an overview of new strategies to combat the drug resistance of Gram-negative bacteria.
作者
王清
韩文宇
李清蓉
赵乐怡
张涵清
郭昀丽
秦有才
梁鑫鑫
范恩国
储引娣
WANG Qing;HAN Wenyu;LI Qingrong;ZHAO Leyi;ZHANG Hanqing;GUO Yunli;QIN Youcai;LIANG Xinxin;FAN Enguo;CHU Yindi(College of Life Sciences,Linyi University,Linyi,Shandong,China;Institute of Basic Medical Sciences Chinese Academy of Medical Sciences,School of Basic Medicine Peking Union Medical College,Beijing,China)
出处
《微生物学报》
北大核心
2025年第12期5244-5256,共13页
Acta Microbiologica Sinica
基金
国家自然科学基金(32200153)。
