摘要
以“中国脑计划”这一国家重大需求为导向,结合电分析化学前沿科研成果—碳纳米电极的制备及其单囊泡原位电分析化学应用,现推出面向本科生的“单囊泡超高分辨电分析化学”开放创新型实验。利用化学、生命科学等多学科交叉实验平台,使用激光拉制仪,采用化学气相沉积法,制备尖端直径大小约为300 nm碳纳米电极;利用循环伏安法和安培法等电化学技术,研究碳纳米电极对神经递质多巴胺的电化学分析性能;进一步将所制备的碳纳米电极应用于PC12细胞单囊泡分泌儿茶酚胺的电化学原位监测。采用“需求导向-实验设计与执行-评价”的思维模式,将学生文献调研、实验方案的设计与执行、评价等多个环节纳入考察范围,将求实创新、科技强国、“西迁精神”等思政元素有效融入实验教学全过程,全面提升学生的自主探究能力、创新思维、实践操作能力以及科学素养,促进了具有解决复杂问题的高质量创新型基础学科人才的培养。
An innovative analytical chemistry experiment is designed to introduce the fabrication of carbon nanoelectrode and their application in in-situ electroanalytical studies of individual vesicles into undergraduate experimental teaching.This experiment consists of three parts:first,carbon nanoelectrodes with tip diameters of 300 nm is fabricated by chemical vapor deposition and laser-assisted pulling method.Subsequently,the electrochemical performance of carbon nanoelectrodes towards dopamine is studied using cyclic voltammetry and amperometric techniques.Finally,in-situ monitoring of catecholamine released from individual synaptic vesicles in PC12 cells is successfully achieved.The innovative experiment teaching process include literature search,experimental design,and discussion.This experiment can fully embody a scientific research thinking mode of“demand-driven,design-execution and evaluation”.This experimental system construction can cultivate the ability of undergraduate students with autonomy,creativity,practical skills,and scientific literacy through the opening of interdisciplinary experimental platforms.Throughout the teaching process,the ideological and political elements such as seeking truth and innovation are integrated to inspire students.This approach fosters the development of innovative talents capable of student to solve complex problems.
作者
漆贺同
杨孙莹
霍靖骁
张军杰
李延
李银环
王昌河
漆红兰
QI Hetong;YANG Sunying;HUO Jingxiao;ZHANG Junjie;LI Yan;LI Yinhuan;WANG Changhe;QI Honglan(School of Chemistry,Xi'an Jiaotong University,Xi'an 710049,China;School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,China;College of Chemistry and Materials Science,Northwest University,Xi'an 710069,China;School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi'an 710062,China)
出处
《实验室科学》
2025年第6期80-87,共8页
Laboratory Science
基金
国家自然科学基金面上项目(项目编号:22474105,22074087)
陕西省本科和高等继续教育教学改革研究项目(项目编号:23BY031)
陕西省重点研发计划项目(项目编号:2024SF-YBXM-161)。
关键词
分析化学
开放创新型实验
碳纳米电极
单囊泡
analytical chemistry
open and innovative experiment
carbon nanopipette electrode
individual vesicles
