摘要
目的:构建一种适配体交联水凝胶荧光生物传感器用于食品中玉米赤霉烯酮(zearalenone,ZEN)的检测。方法:引物A链和修饰淬灭基团BHQ1的引物B链在一定条件下发生共聚形成聚合物A(PS-A)和聚合物B(BHQ1-PS-B),修饰有6-羧基荧光素(6-carboxyfluorescein,FAM)荧光基团的核酸适配体(aptamers,Apt)(FAM-Apt)作为信号探针和交联剂与PS-A和BHQ1-PS-B通过碱基互补配对交联形成DNA水凝胶。在ZEN存在的情况下,ZEN与BHQ1-PS-B竞争结合FAM-Apt,水凝胶裂解,荧光基团和淬灭基团由距离很近变为相互分离状态,荧光信号“开启”。结果:该方法可实现ZEN一步定性和定量分析,线性范围为1~500 nmol/L,检出限为8.7 nmol/L。面粉样品的加标回收率为80.3%~94.2%。结论:所建立的适配体交联水凝胶荧光生物传感器具有灵敏高效、检测时间短等优势,可应用于食品中ZEN的快速检测。
Objective:To develop an aptamer-crosslinked fluorescent hydrogel biosensor for the detection of zearalenone(ZEN)in foods.Methods:Primer strand A and strand B modified with Black Hole Quencher 1(BHQ1)were copolymerized under specific conditions to form polymer A(PS-A)and polymer B(BHQ1-PS-B),respectively.Aptamer labeled with 6-carboxyfluorescein(FAM-Apt),serving as both a signal probe and crosslinker,hybridized with PS-A and BHQ1-PS-B via complementary base pairing to form a DNA hydrogel.ZEN competed with BHQ1-PS-B for binding to FAM-Apt,leading to hydrogel dissociation.This resulted in the separation of the fluorophore(FAM)and the quencher(BHQ1),turning on the fluorescence signal.Results:This method enabled one-step qualitative and quantitative analysis of ZEN,with a linear range of 1–500 nmol/L and a detection limit of 8.7 nmol/L.Recovery rates for spiked flour samples ranged from 80.3%to 94.2%.Conclusion:The aptamer-crosslinked fluorescent hydrogel biosensor is sensitive,efficient,time-saving,and thus suitable for the rapid detection of ZEN in foods.
作者
李可敬
谢刚
窦金鑫
张鸿鹏
罗菲
郭玉垚
韩逸陶
LI Kejing;XIE Gang;DOU Jinxin;ZHANG Hongpeng;LUO Fei;GUO Yuyao;HAN Yitao(Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《食品科学》
北大核心
2025年第24期65-71,共7页
Food Science
基金
“十四五”国家重点研发计划重点专项(2023YFF1104603-03)
中央级公益性科研院所基本科研业务费专项(ZX2434)。
