摘要
【目的】为探索百合LhSorPR10-1在抗灰霉病中的作用,克隆LhSorPR10-1,分析其进化关系、编码蛋白的理化性质等。【方法】构建了原核表达载体,对重组蛋白进行了诱导表达,进行了体外抑菌活性及核酸酶活性分析;构建了植物超表达及反义表达载体并转入“索邦”百合中,并对转基因百合进行抗病性分析。【结果】LhSorPR10-1为稳定的亲水性蛋白,定位在细胞质中,与岷江百合的亲缘关系最高。LhSorPR10-1重组蛋白对椭圆葡萄孢菌有显著的抑制作用,且具有RNase和DNase活性。百合中过表达LhSorPR10-1基因的转基因株系在染病后相关抗氧化酶活性、木质素和脯氨酸含量等都比对照和抑制表达株系更高,提高了百合灰霉病的抗病性。【结论】LhSorPR10-1基因在百合抗灰霉病中起重要作用。
【Objective】To explore the role of lily LhSorPR10-1 in resistance to gray mold,LhSorPR10-1 was cloned and its evolutionary relationship,as well as the physicochemical properties of the encoded protein,were analyzed.【Method】We constructed a prokaryotic expression vector,induced the expression of the recombinant protein,and analyzed antibacterial activity and nuclease activity in vitro.We constructed plant over-expression and antisense expression vectors and transferred them into the'Sorbonne'lily,and evaluated the disease resistance of the transgenic lily.【Result】The results showed that LhSorPR10-1 is a stable hydrophilic protein located in the cytoplasm and has the highest phylogenetic relationship with Lilium regale.The recombinant protein LhSorPR10-1 has a significant inhibitory effect on Botrytis elliptica and exhibits both RNase and DNase activity.The transgenic lines of overexpressing LhSorPR10-1 in lily showed higher relevant antioxidant enzyme activities,lignin and proline contents after infection compared to the control and the LhSorPR10-1 suppressed expression lines,improving the resistance of lily to gray mold.【Conclusion】LhSorPR10-1 plays an important role in gray mold resistance in lily.
作者
王慧茹
邹雨
刘思娴
杜文婷
罗兰
眭顺照
刘道凤
WANG Huiru;ZOU Yu;LIU Sixian;DU Wenting;LUO Lan;SUI Shunzhao;LIU Daofeng(College of Horticulture and Landscape Architecture,Southwest University/Key Laboratory of Horticulture Science for Southern/Mountainous Regions of Ministry of Education/Chongqing Engineering Research Center for Floriculture,Chongqing 400715,China;Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences,Chongqing 400722,China)
出处
《四川农业大学学报》
北大核心
2025年第6期1584-1594,共11页
Journal of Sichuan Agricultural University
基金
重庆市研究生科研创新项目(CYS22222)
福建省中科院STS计划配套项目(2022T3031)。
关键词
百合
病程相关蛋白
抑菌活性
核酸酶活性
抗病性
lily
pathogenesis related protein
antibacterial activity
nuclease activity
disease resistance
