摘要
目的探讨河北燕达医院2025年1月1日—2025年6月30日耳念珠菌的菌种鉴定和药物敏感性以及基因组特征,为制定院内感染防控策略提供依据。方法对2例耳念珠菌进行病例溯源,通过初筛鉴定结合MALDITOF MS质谱技术进行复核确认;采用Sensititre YeastOne检测抗真菌药物的最低抑菌浓度,并进行基因组测序分析。结果初筛鉴定为希木龙念珠菌和无名念珠菌,经Vitek MS质谱验证确认为耳念珠菌。药敏试验表明两株菌对氟康唑呈现显著耐药性,均与南亚分支B8441(CladeⅠ)型具有高度的相似性和耐药基因ERG11_Y132F、TAC1B_A583S和FCY1_S70R的变异。结论该研究揭示了河北燕达医院耳念珠菌的耐药性特征及分子溯源,为南亚分支氟康唑耐药耳念珠菌,临床早期预警机制建立和诊疗方案优化提供了科学依据。
Objective To investigate the species identification,drug sensitivity and genomic characteristics of Candida auris in Hebei Yanda Hospital from January 1,2025 to June 30,2025,and to provide a basis for formulating infection control strategies within the hospital.Methods Two cases of C.auris were traced back to their sources.The initial identification was confirmed by MALDI-TOF MS mass spectrometry.The minimum inhibitory concentrations of antifungal drugs were detected using Sensititre YeastOne,and genomic sequencing analysis was conducted.Results The initial identification was C.haemulonii and C.famata,which were confirmed as C.auris by Vitek MS mass spectrometry.The drug sensitivity test showed that both strains were significantly resistant to fluconazole,and they were highly similar to the South Asian clade B8441(Clade I)type and had variations in the drug resistance genes ERG11_Y132F,TAC1B_A583S and FCY1_S70R.Conclusions This study reveals the drug resistance characteristics and molecular traceability of C.auris in Hebei Yanda Hospital,which is a South Asian clade B8441(Clade I)type resistant to fluconazole.It provides a scientific basis for establishing an early warning mechanism and optimizing the diagnosis and treatment plan in clinical practice.
作者
栗方
张艳利
王娟
王诗淇
张浩
王金菊
王军爽
LI Fang;ZHANG Yanli;WANG Juan;WANG Shiqi;ZHANG Hao;WANG Jinju;WANG Junshuang(Department of Infection and Disease Control,Hebei Yanda Hospital,Hebei Langfang 065201,China;Department of Infection and Clinical Microbiology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China;Department of Critical Care Medicine,Hebei Yanda Hospital,Hebei Langfang 065201,China;Department of Laboratory Medicine,Hebei Yanda Hospital,Hebei Langfang 065201,China;Department of Scientific Research and Teaching,Hebei Yanda Hospital,Hebei Langfang 065201,China)
出处
《中国真菌学杂志》
2025年第6期594-598,共5页
Chinese Journal of Mycology
基金
河北省医学科学研究课题计划项目(20261154)。
关键词
耳念珠菌
流行特征
质谱检测
耐药性
Candida auris
epidemiological characteristics
mass spectrometry validation
drug resistance
