摘要
目的分析口腔鳞状细胞癌(OSCC)组织中基础转录因子3(BTF3)、趋化因子配体5(CXCL5)的表达及其与病理特征、预后的关系。方法选取2021年2月至2024年2月于泰州市第二人民医院手术治疗的81例OSCC患者临床资料,收集患者癌组织及癌旁组织标本,分别采用肿瘤基因组图谱数据库、免疫组化酶标聚合物法检测上述组织内BTF3、CXCL5的表达情况并对比,采用Kaplan-Meier曲线分析不同BTF3、CXCL5表达的OSCC患者生存情况;采用单因素及多因素Logistic分析影响OSCC患者预后的危险因素,采用Hosmer-Lemeshoe检验OSCC患者预后模型的拟合优度。结果OSCC癌组织中BTF3、CXCL5阳性表达率均高于癌旁组织,差异有统计学意义(P<0.05);淋巴结转移、中低分化患者BTF3、CXCL5阳性表达率均高于无淋巴结转移及高分化患者,差异有统计学意义(P<0.05);Kaplan-Meier曲线分析结果显示,BTF3、CXCL5阳性患者的中位生存时间(OS)均短于BTF3、CXCL5阴性患者,差异有统计学意义(Log-Rankχ^(2)=5.711、4.111,P=0.017、0.043);预后不良组发生淋巴结转移、中低分化、BTF3阳性、CXCL5阳性患者例数占比均高于预后良好组,差异有统计学意义(P<0.05);多因素Logistic分析结果显示,发生淋巴结转移(OR=3.655)、BTF3阳性(OR=4.133)、CXCL5阳性(OR=4.491)是影响OSCC患者预后的危险因素(P<0.05),OSCC患者预后模型的拟合优度检验较优(Hosmer-Lemeshoe P=0.715)。结论BTF3、CXCL5在OSCC癌组织中呈阳性,且其表达与病情进展及不良预后密切相关。
Objective To analyze the expressions of basal transcription factor 3(BTF3)and C-X-C motif chemokine ligand 5(CXCL5)in oral squamous cell carcinoma(OSCC)tissues and their relationship with pathological features and prognosis.Methods The clinical data of 81 patients with OSCC who received surgical treatment at Taizhou Second People's Hospital from February 2021 to February 2024 were selected.Cancer tissues and adjacent tissues were collected as specimens.The expressions of BTF3 and CXCL5 in these tissues were detected and compared using the tumor genome atlas database and immunohistochemical enzyme-la-beled polymer method.Kaplan-Meier curve was used to analyze the survival status of OSCC patients with differ-ent BTF3 and CXCL5 expressions.Univariate and multivariate logistic analyses were applied to analyze the risk factors affecting the prognosis of OSCC patients.Hosmer-Lemeshoe was used to test the goodness-of-fit of the prognosis model of OSCC patients.Results The positive expression rates of BTF3 and CXCL5 in OSCC can-cer tissues were higher than those in adjacent tissues,with statistical differences(P<0.05).The positive expres-sion rates of BTF3 and CXCL5 in patients with lymph node metastasis and moderate-to-low differentiation were higher than those in patients without lymph node metastasis and patients with high differentiation,with statisti-cal differences(P<0.05).Kaplan-Meier curve analysis showed that the median survival time(OS)of patients with positive BTF3 or CXCL5 was shorter than that of patients with negative BTF3 or CXCL5 respectively(Log-Rankχ^(2)=5.711,4.111,P=0.017,0.043).The proportions of patients with lymph node metastasis,moder-ate-to-low differentiation,positive BTF3 and positive CXCL5 in the poor prognosis group were higher than those in the good prognosis group,and the differences were statistically significant(P<0.05).Multivariate logis-tic analysis showed that lymph node metastasis(OR=3.655),positive BTF3(OR=4.133)and positive CXCL5(OR=4.491)were risk factors affecting the prognosis of OSCC patients(P<0.05).The goodness-of-fit test for the prognosis model of OSCC patients was better(Hosmer-Lemeshoe P=0.715).Conclusion BTF3 and CXCL5 are both positively expressed in OSCC cancer tissues,and their levels are closely associated with dis-ease progression and a poor prognosis.
作者
陈华荣
李敏
蒋佳宇
CHEN Huarong;LI Min;JIANG Jiayu(Department of Stomatology,Taizhou Second People's Hospital,Taizhou,Jiangsu,China,225500;Blood Evolution Center,Taizhou Second People's Hospital,Taizhou,Jiangsu,China,225500)
出处
《分子诊断与治疗杂志》
2025年第12期2306-2309,2314,共5页
Journal of Molecular Diagnostics and Therapy
基金
江苏省科技项目(ZY202311024)。
关键词
口腔鳞状细胞癌
基础转录因子3
趋化因子配体5
Oral squamous cell carcinoma
Basic transcription factor 3
C-X-C motif chemokine ligand 5
