摘要
40 Hz光闪烁刺激被认为在阿尔茨海默病(AD)的治疗中具有潜在应用价值。但是,其长期作用是否能够改善工作记忆及其相关机制仍有待进一步探索。本研究选用21只成年Wistar大鼠,随机分为AD光刺激组、AD组与对照组。对前两组大鼠进行AD模型制备,并对AD光刺激组进行40 Hz光闪烁长期作用。随后,基于T迷宫任务评估各组大鼠工作记忆功能,并从海马(HPC)和前额叶皮层(mPFC)神经元兴奋性和脑区间神经电活动同步性变化的角度,探索40 Hz光刺激影响工作记忆的潜在神经机制。结果显示,40 Hz光闪烁长期作用改善了AD大鼠的工作记忆功能,增强了HPC-mPFC间神经活动的同步性,以及两脑区之间θ和低频γ振荡介导的信息传递水平。该研究为探索40 Hz光闪烁刺激的作用机制及其在AD疾病防治中进一步的临床应用提供了一定的理论支持。
40 Hz light flicker stimulation is deemed to hold considerable promise in the treatment of Alzheimer’s disease(AD).However,whether its long-term effect can improve working memory and its related mechanisms remains to be further explored.In this study,21 adult Wistar rats were randomly divided into the AD light-stimulation group,the AD group and the control group.AD models were established in the first two of these groups,with the light-stimulation group receiving long-term 40 Hz light flicker stimulation.Working memory performance across groups was subsequently evaluated using the T-maze task.To investigate the potential neural mechanisms underlying the effects of 40 Hz light stimulation on working memory,we examined changes in neuronal excitability within the hippocampus(HPC)and medial prefrontal cortex(mPFC),as well as alterations in inter-regional synchronization of neural activity.The findings demonstrated that prolonged 40 Hz light stimulation significantly improved working memory performance in AD model rats.Furthermore,the intervention enhanced the synchronization of neural activity between the hippocampus(HPC)and medial prefrontal cortex(mPFC),as well as the efficiency of information transfer,primarily mediated by theta and low-frequency gamma oscillations.This study provides theoretical support for exploring the mechanisms of 40 Hz light flicker stimulation and its further clinical application in the prevention and treatment of Alzheimer’s disease.
作者
刘素红
王龙龙
李双燕
徐桂芝
LIU Suhong;WANG Longlong;LI Shuangyan;XU Guizhi(School of Health Science and Biomedical Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;Hebei Key Laboratory of Bioelectromagnetic and Neural Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;The State Key Laboratory of Reliability and Intelligence of Electrical Equipment,Hebei University of Technology,Tianjin 300130,P.R.China)
出处
《生物医学工程学杂志》
北大核心
2025年第6期1107-1114,共8页
Journal of Biomedical Engineering
基金
国家自然科学基金(51737003,5150704)
河北省自然科学基金(F2018202319)
河北省引进留学人员资助项目(C20200315)。
关键词
40
Hz光闪烁刺激
阿尔茨海默病
神经电活动特性
工作记忆
海马
前额叶
40 Hz light flicker
Alzheimer’s disease
Neural electrical activity characteristics
Working memory
Hippocampus
Prefrontal lobe
