摘要
旨在探讨抗性砧木嫁接提高番茄枯萎病抗性的生理机制,为应用嫁接提高番茄枯萎病抗性提供理论依据。以番茄抗病材料‘FR’为砧木,感病材料‘AC’为接穗,进行感病自根嫁接(自根苗)和感病抗性砧木嫁接(嫁接苗),测定尖孢镰刀菌侵染后两类嫁接苗的发病情况、光合作用、抗氧化酶活性、激素含量及相关基因表达量。结果表明:番茄嫁接苗枯萎病病情指数和发病率比自根苗分别显著降低95.6%和90.7%。在尖孢镰刀菌侵染后,嫁接苗的净光合速率、气孔导度、蒸腾速率、最大光化学效率等光合荧光参数,茉莉酸含量和茉莉酸信号抑制因子的表达量显著高于自根苗,其SOD、POD、CAT和APX活性显著低于自根苗,水杨酸和氨基环丙烷羧酸含量与自根苗没有显著差异,但水杨酸和乙烯信号通路上的基因表达量均高于自根苗。番茄抗病砧木嫁接主要通过提高叶片光合效率、调节抗氧酶活性和信号转导获得系统抗性的方式显著提高植株对枯萎病的抗性。
The study aims to explore the effects of Fusarium oxysporum infection on physiological and biochemical indices of the tomato seedlings grafted with resistant rootstock,in order to provide theoretical basis for understanding the mechanism of grafting in improving tomato resistance to Fusarium wilt.The disease-resistant tomato‘FR’was used as rootstock and the susceptible tomato‘AC’as scion.Grafting was conducted onto the resistant rootstock and self-rooted plants of the susceptible materials.Pathogenesis,photosynthesis,antioxidant enzyme activity,hormone content,and gene expression were measured in both of the grafted plants following infection with F.oxysporum.Compared with the self-rooted plants,the grafted tomato plants were reduced by 95.6% and 90.7%,respectively,in the disease index and incidence of Fusarium wilt disease.After F.oxysporum infection,the grafted plants exhibited higher photosynthetic parameters,including net photosynthetic rate,stomatal conductance,transpiration rate,and maximum photochemical efficiency,as well as increased jasmonic acid content and expression of genes in the JA signaling pathway,compared to the self-rooted plants.The activities of SOD,POD,CAT,and APX in grafted plants were lower than those in the self-rooted plants,while salicylic acid and aminocyclopropane-1-carboxylate levels showed no significant differences.However,the expression of genes in the SA and ET signaling pathways was higher in grafted plants than in self-rooted plants.Grafting onto resistant rootstocks enhances tomato resistance to Fusarium wilt,which is achieved primarily through the improvement of leaf photosynthetic efficiency,regulation of antioxidant enzyme activity,and activation of systemic resistance via signaling pathways.
作者
卢盼玲
玄立杰
张红梅
田守波
何锴
张辉
LU Panling;XUAN Lijie;ZHANG Hongmei;TIAN Shoubo;HE Kai;ZHANG Hui(Shanghai Key Laboratory of Facility Horticulture Technology,Institute of Horticulture,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Jiading District Agricultural Technology Extension Service Center,Shanghai 201106,China)
出处
《西北农业学报》
北大核心
2025年第12期2322-2330,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
上海市科技兴农项目(2022-02-08-00-12-F01092)。
关键词
番茄枯萎病
嫁接
尖孢镰刀菌
光合作用
抗氧化酶
激素
转录组
Tomato wilt
Grafting
Fusarium oxysporum
Photosynthesis
Antioxidant enzymes
Hormone
Transcriptome
