摘要
双酚AF(bisphenol AF,BPAF)是一种常见的内分泌干扰物,广泛应用于工业生产和日常消费品中。环境监测显示,BPAF在水体、土壤以及生物体内普遍存在,并对生态系统可能造成潜在危害。然而,关于BPAF对两栖类生物神经毒性的研究尚不充分。本研究旨在评估BPAF对非洲爪蟾(Xenopus laevis)蝌蚪的生长发育、神经递质水平、运动行为及相关基因表达的影响。实验结果表明,BPAF暴露促进了蝌蚪的生长发育,尤其在30μg·L^(-1)高浓度暴露组中,蝌蚪的后肢长度和发育进程显著增加。化学分析表明,BPAF在蝌蚪大脑中具有显著的浓度依赖性富集效应,并伴随神经系统的损害,表现为蝌蚪大脑中神经递质GABA和其他神经递质合成代谢相关物质胆碱、色胺和酪胺含量的增加。此外,BPAF还显著抑制了发育相关基因(如ccne、neurod和igf)的表达,并降低了蝌蚪的运动速率。值得关注的是,本研究中BPAF对蝌蚪发育时期,GABA、胆碱、色胺和酪胺含量以及运动速率等终点指标的最大无观察效应浓度均为3μg·L^(-1)。综上,BPAF不仅干扰蝌蚪的早期发育,更通过扰乱神经递质稳态,对其神经功能与行为调控产生负面影响,这为BPAF作为内分泌干扰物对两栖类生物的潜在神经毒性提供了有力证据。研究为评估BPAF对水生生态系统的潜在风险提供了重要数据。
Bisphenol AF(BPAF),a common endocrine-disrupting chemical,is widely used in industrial production and consumer products.Environmental monitoring has shown that BPAF is ubiquitously present in aquatic ecosystems,soils,and biota,potentially posing significant ecological risks.However,research on the neurotoxicity of BPAF in amphibians remains limited.The present study aimed to evaluate the effects of BPAF exposure on the growth,d evelopment,neurotransmitter levels,locomotor behavior,and related gene expression in Xenopus laevis tadpoles.The results revealed that BPAF exposure promoted tadpole growth,particularly at a high concentration of 30μg·L^(-1),where significant increases in hind limb length and developmental stage were observed.Chemical analysis showed that BPAF accumulated in tadpole brain tissue in a concentration-dependent manner,accompanied by n eurotoxic effects,as evidenced by increased levels of the neurotransmitters GABA and other neurotransmitter s ynthesis-related metabolites,including choline,tryptamine,and tyramine.Additionally,exposure to BPAF significantly inhibited the expression of developmental genes(such as ccne,neurod,and igf)and reduced tadpole locomotor activity.It is noteworthy that the no-observed-effect concentration(NOEC)for all endpoints examined in this study,including developmental stage,GABA level,and contents of choline,tryptamine,tyramine,as well as swimming speed,was consistently determined to be 3μg·L^(-1)in tadpoles exposed to BPAF.In summary,BPAF not only disrupted the early development of tadpoles but also adversely affected their neural function and behavioral regulation by disturbing neurotransmitter homeostasis.These findings provided compelling evidence for the p otential neurotoxicity of BPAF as an endocrine-disrupting chemical in amphibians and contributed important data for assessing the potential risks of BPAF in aquatic ecosystems.
作者
李芳
毋晗
曹苗苗
冯军厂
王先锋
LI Fang;WU Han;CAO Miaomiao;FENG Junchang;WANG Xianfeng(College of Fisheries,Henan Normal University,Xinxiang 453007,China;Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province,Nanyang 474450,China)
出处
《生态毒理学报》
北大核心
2025年第6期25-34,共10页
Asian Journal of Ecotoxicology
基金
国家自然科学基金青年基金项目(21906046)
河南省自然科学基金面上基金项目(242300420196,252300420199)。
关键词
双酚AF
非洲爪蟾
神经递质
发育
行为
bisphenol AF
Xenopus laevis
neurotransmitters
development
behavior
