摘要
本研究对75份二倍体马铃薯栽培种PHU-STN杂种后代无性系组培苗进行抗旱棚控水种植,测定其在干旱胁迫下的生物量、根系相关指标和生理指标等7个性状表征值,计算各指标抗旱胁迫指数,并利用综合隶属函数法进行主成分分析和聚类分析,将所有材料分为4个抗旱等级,筛选出高抗旱材料2份(A90、A204)。以A90(高抗旱性)与A163(干旱敏感)为试验材料,利用转录组测序技术研究抗旱性差异较大的二倍体马铃薯材料在PEG-6000胁迫下的差异表达基因,基于差异表达基因的GO功能注释和KEGG通路富集分析,利用生长素与乙烯信号传导途径的关键节点基因差异表达水平变化量和相关基因qRT-PCR表达量初步筛选到6个抗旱候选基因,这些基因均与生长素和乙烯信号传导通路相关。
In this study,drought-shelter controlled irrigation experiments were conducted on 75 tissue-cultured seedling clones derived from hybrid progeny of the diploid cultivated species PHU-STN.Seven traits,measurements including biomass,root-related indicators,and physiological indices under drought stress,were measured for each potato accession.Drought stress indices were calculated for each trait,and a comprehensive evaluation was performed using membership function analysis combined with principal component and cluster analyses.Based on the results,the materials were classified into four levels of drought resistance,and two highly drought-resistant clones(A90 and A204)were identified.Subsequently,A90(high drought resistance)and A163(low drought resistance)were subjected to PEG-6000-induced drought stress for sampling and analysis.Transcriptome sequencing was employed to explore the regulatory mechanisms underlying drought response and to identify drought-resistancerelated genes.Differentially expressed genes(DEGs)were analyzed in A90 and A163 under PEG-6000 stress.GO functional annotation and KEGG pathway enrichment analyses revealed key regulatory genes involved in ethylene and auxin signaling pathways.Based on differential expression patterns and qRT-PCR validation,six candidate drought-resistance genes were identified,all associated with auxin and ethylene signaling pathways.
作者
王雅致
杨飚
季香林
石瑛
张丽莉
Wang Ya-Zhi;Yang Biao;Ji Xiang-Lin;Shi Ying;Zhang Li-Li(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)
出处
《作物学报》
北大核心
2026年第1期72-84,共13页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31601355)
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09)资助。
关键词
马铃薯
二倍体
干旱胁迫
综合评价
抗旱候选基因
potato
diploid
drought stress
comprehensive evaluation
drought resistant candidate genes
