摘要
采用MRS-CaCO3琼脂培养基从青海省尖扎县传统发酵牦牛乳中分离得到3株疑似乳酸菌分别编号为J1、J2、J3,以3株商业化标准菌株(Lactobacillus casei C1、Streptococcus salivarius subsp.thermophilus C2、Pediococcus acidilactici C3)为参照,对其进行生理生化鉴定、分子生物学鉴定。结果表明,3株乳酸菌J1、J2、J3分别为粪肠球菌(Enterococcus faecium J1)、植物乳植杆菌(Lactiplantibacillus plantarum J2)、面包乳杆菌(Lactobacillus crustorum J3)。通过对其进行葡萄糖产酸产气、生长曲线、产酸曲线、耐酸与耐胆盐性能、疏水性、自凝集性与安全性等体外益生特性检测,E.faecium J1、L.plantarum J2、L.crustorum J3均有良好的体外益生特性,进一步的发酵特性检测及综合分析结果表明菌株L.crustorum J3可作为潜在的乳用基础发酵剂。
The author isolated three lactic acid bacteria strains from traditional fermented yak milk using MRS-CaCO3 agar medium.They analyzed these strains alongside three standard commercial strains for physiological and molecular characteristics.Results indicated that strains J1,J2,and J3 are Enterococcus faecium J1,Lactiplantibacillus plantarum J2,and Lactobacillus crustorum J3,respectively.All strains exhibited favorable in vitro probiotic properties.L.crustorum J3 displayed excellent fermentation characteristics,suggesting its potential as a dairy fermentation agent.
作者
姜洋洋
佟海峰
罗璇
张剑搏
吴国芳
王磊
JIANG Yangyang;TONG Haifeng;LUO Xuan;ZHANG Jianbo;WU Guofang;WANG Lei(College of Animal Science and Veterinary Science,Qinghai University,Xining 810016,China;Qinghai Plateau Grazing Livestock Key Laboratory of Animal Nutrition and Feed Science,Xining 810016,China;Key Laboratory of Livestock and Poultry Genetics and Breeding on Qinghai-Tibet Plateau,Ministry of Agriculture and Rural Affairs,Xining 810016,China;Qinghai Plateau Key Laboratory of Animal Genetic Resources Conservation and Innovative Utilization,Xining 810016,China)
出处
《中国乳品工业》
北大核心
2025年第12期29-37,共9页
China Dairy Industry
基金
青海省科技成果转移转化项目(2022ZY008)。
关键词
牦牛乳源
乳酸菌
分离
鉴定
益生特性
发酵特性
yak milk source
lactic acid bacteria
isolation
identification
probiotic characteristics
fermentation characteristics
