摘要
目的比较生草乌及诃子汤制草乌的急性毒性、抗炎、镇痛作用。方法急性毒性试验分为空白对照组[A组,0.5%羧甲基纤维素钠(CMC-Na)],生草乌1.67 g/kg剂量组(B_(1)组)、2.22 g/kg剂量组(B_(2)组)、2.96 g/kg剂量组(B_(3)组)、3.95 g/kg剂量组(B_(4)组)、5.26 g/kg剂量组(B_(5)组)、7.01 g/kg剂量组(B6组),诃子汤制草乌组(C组,8.40 g/kg),各10只。各组小鼠按每10 g体质量单次灌胃相应药物0.4 mL,给药2次,间隔12 h,之后常规饲养14 d。考察小鼠的死亡情况、半数致死量(LD50)、体质量及血清乳酸脱氢酶(LDH)、肌酸激酶(CK)、碱性磷酸酶(ALP)、总胆汁酸(TBA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平。抗炎、镇痛药效试验分为模型对照组(D组,等量0.5%CMC-Na),西药对照组(E组,对乙酰氨基酚0.250 g/kg),生草乌低、中、高剂量组(F_(1)组、F_(2)组、F_(3)组,0.114,0.171,0.342 g/kg),诃子汤制草乌低、中、高剂量组(G_(1)组、G_(2)组、G_(3)组,0.114,0.171,0.342 g/kg),各10只。各组小鼠均按每10 g体质量单次灌胃相应药物0.4 mL,分别进行醋酸扭体试验、热板试验、二甲苯致耳肿胀试验,考察小鼠发生扭体反应的潜伏期及发生频次、疼痛阈值、耳肿胀度及耳组织病理学变化。结果急性毒性试验结果显示,C组、B_(1)组小鼠均未死亡,B6组小鼠均死亡,生草乌的LD50[95%置信区间(95%CI)]为3.4214(2.8523,4.1039)g/kg;与C组比较,B_(3)组小鼠给药后第5,10,14天及B_(4)组小鼠给药后第10,14天的体质量均显著升高(P<0.05),B_(4)组、B_(5)组小鼠给药后第5天的体质量均显著降低(P<0.05);B_(1)组、B_(3)组、B_(4)组、B_(5)组小鼠的ALT和AST水平均有显著差异(P<0.01);B_(1)组、B_(2)组、B_(4)组、B_(5)组小鼠的TBA水平均有显著差异(P<0.01);B_(1)组、B_(2)组、B_(3)组、B_(4)组、B_(5)组小鼠的ALP,CK,LDH水平均有显著差异(P<0.01)。抗炎、镇痛药效试验结果显示,与D组比较,F_(1)组、F_(2)组、F_(3)组、G_(1)组、G_(2)组、G_(3)组小鼠第1次发生扭体反应的潜伏期均显著延长(P<0.05),F_(3)组、G_(1)组、G_(2)组、G_(3)组小鼠的扭体反应发生频次均显著降低(P<0.01);F_(1)组、F_(2)组、F_(3)组、G_(1)组、G_(2)组、G_(3)组小鼠给药后30,60,90,120 min的疼痛阈值均显著升高(P<0.01);F_(1)组、F_(2)组、F_(3)组、G_(1)组、G_(2)组、G_(3)组小鼠的耳肿胀度均显著降低(P<0.01)。生草乌与诃子汤制草乌相同剂量组间比较,F_(1)组、F_(2)组小鼠第1次发生扭体反应的潜伏期分别较G_(1)组、G_(2)组显著缩短(P<0.05),F_(1)组、F_(2)组、F_(3)组小鼠的扭体反应发生频次分别较G_(1)组、G_(2)组、G_(3)组显著升高(P<0.05);F_(1)组、F_(2)组、F_(3)组小鼠给药60,90 min后的疼痛阈值分别较G_(1)组、G_(2)组、G_(3)组显著降低(P<0.05),F_(2)组、F_(3)组小鼠给药120 min后的疼痛阈值分别较G_(2)组、G_(3)组显著降低(P<0.05);各给药组间小鼠耳肿胀度无显著差异(P>0.05)。结论生草乌及诃子汤制草乌均有一定抗炎、镇痛作用,生草乌经诃子汤炮制后具有减毒增效作用。
Objective To compare the acute toxicity,anti-inflammatory,and analgesic effects of raw Aconiti Kusnezoffii Radix and Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction.Methods The acute toxicity test was divided into the blank control group[group A,0.5%carboxymethyl cellulose sodium(CMC-Na)],raw Aconiti Kusnezoffii Radix 1.67 g/kg dose group(group B_(1)),2.22 g/kg dose group(group B_(2)),2.96 g/kg dose group(group B_(3)),3.95 g/kg dose group(group B_(4)),5.26 g/kg dose group(group B_(5)),7.01 g/kg dose group(group B6),and Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction(group C,8.40 g/kg),with 10 rats in each group.Mice in each group were intragastrically administered the corresponding drugs at a volume of 0.4 mL per 10 g body weight,twice with a 12-hour interval.All mice were fed under conventional conditions for 14 d.The mortality rate,median lethal dose(LD50),body mass,and serum levels of lactate dehydrogenase(LDH),creatine kinase(CK),alkaline phosphatase(ALP),total bile acid(TBA),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)were investigated.The anti-inflammatory and analgesic efficacy tests were divided into the model control group(group D,equal volume of 0.5%CMC-Na),the Western medicine control group(group E,0.250 g/kg of acetaminophen),low-,medium-,and high-dose groups of raw Aconiti Kusnezoffii Radix(groups F_(1),F_(2),F_(3),0.114,0.171,0.342 g/kg),and low-,medium-,and high-dose groups of Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction(groups G_(1),G_(2),G_(3),0.114,0.171,0.342 g/kg),with 10 rats in each group.Mice in each group were intragastrically administered the corresponding drugs at a volume of 0.4 mL per 10 g body weight.Acetic acid writhing test,hot plate test,and xylene-induced ear swelling test were performed to investigate the latency to the first writhing response,writhing frequency,pain threshold,ear swelling degree,and pathological changes in ear tissue.Results The acute toxicity test results showed that no mice in groups C and B_(1)died,and all mice in group B6 died.The LD50[95%confidence interval(95%CI)]of raw Aconiti Kusnezoffii Radix was 3.4214(2.8523,4.1039)g/kg.Compared with those in group C,the body mass in groups B_(3)on the 5th,10th,14th days and that in group B_(4)on the 10th,14th days after drug administration significantly increased(P<0.05),while the body mass in groups B_(4)and B_(5)significantly decreased on the 5th day after drug administration(P<0.05);the ALT and AST levels in groups B_(1),B_(3),B_(4),and B_(5)showed significant differences(P<0.01);the TBA levels in groups B_(1),B_(2),B_(4),and B_(5)showed significant differences(P<0.01);the ALP,CK,and LDH levels in groups B_(1),B_(2),B_(3),B_(4),and B_(5)showed significant differences(P<0.01).The results of anti-inflammatory and analgesic efficacy tests showed that compared with those in group D,the latency to the first writhing response in groups F_(1),F_(2),F_(3),G_(1),G_(2),and G_(3)significantly prolonged(P<0.05),while the writhing frequency in groups F_(3),G_(1),G_(2),and G_(3)significantly reduced(P<0.01);the pain thresholds in groups F_(1),F_(2),F_(3),G_(1),G_(2),and G_(3)significantly increased at 30,60,90,and 120 min after drug administration(P<0.01);the ear swelling in groups F_(1),F_(2),F_(3),G_(1),G_(2),and G_(3)significantly reduced(P<0.01).The comparison between the same dose groups of raw Aconiti Kusnezoffii Radix and Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction showed that the latency to the first writhing response in groups F_(1)and F_(2)significantly shortened compared with that in groups G_(1)and G_(2)(P<0.05),while the writhing frequency in groups F_(1),F_(2),and F_(3)significantly increased compared with that in groups G_(1),G_(2),and G_(3)(P<0.05);the pain thresholds in groups F_(1),F_(2),and F_(3)at 60 and 90 min after drug administration significantly decreased compared with those in groups G_(1),G_(2),and G_(3)(P<0.05);the pain thresholds in groups F_(2)and F_(3)at 120 min after drug administration significantly decreased compared with those in groups G_(2)and G_(3)(P<0.05);there was no significant difference in ear swelling between the treatment groups(P>0.05).Conclusion Both raw Aconiti Kusnezoffii Radix and Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction have anti-inflammatory and analgesic effects.After processing with Chebulae Fructus Decoction,Aconiti Kusnezoffii Radix has a attenuation-preservation effect.
作者
萨日娜
图雅
美丽
陈格歌
巴雅斯拉
包永昌
特日根
松林
Sarina;Tuya;Meili;CHEN Gege;Bayasila;BAO Yongchang;Terigen;Songlin(Inner Mongolia Medical University,Hohhot,Inner Mongolia 010110,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国药业》
2026年第1期54-61,共8页
China Pharmaceuticals
基金
国家自然科学基金[82360848]
内蒙古医科大学重点项目[YKD2023ZD009]。
关键词
生草乌
诃子汤制草乌
急性毒性作用
抗炎作用
镇痛作用
小鼠
raw Aconiti Kusnezoffii Radix
Aconiti Kusnezoffii Radix processed with Chebulae Fructus Decoction
acute toxic effect
anti-inflammatory effect
analgesic effect
mice
