摘要
Nuclear magnetic resonance(NMR)spectroscopy is an excellent tool for simultaneous identification and quantification of metabolites(metabolomics).NMR quantification of human lipoprotein subfractions and their components has proven to be a powerful approach to reveal pathophysiological insights in numerous diseases[1,2].This method has now been standardized with excellent inter-laboratory reproducibility within 10 days for simultaneous quantification of 105 lipoprotein components and 24 low-molecular weight(LMW)metabolites[3];close clustering was also shown for 12 quality-control(QC)samples measured in 3 months although without statistical details[2].However,these reports[[1],[2],[3]]did not cover many vital parameters for lipoprotein components(e.g.,cholesterol-esters and total-lipids),fatty acids,and N-acetyl-glycoproteins(NAGs).
基金
financial supports from the National Key R&D Program of China(Grant Nos.:2022YFC3400700 and 2022YFA0806400)
the Shanghai Municipal Science and Technology Major Project,China(Grant No.:2017SHZDZX01)
the National Natural Science Foundation of China(Grant No.:31821002).
