摘要
目的为探讨小补心汤总黄酮(XBXT-2)对前额叶皮质的神经网络活动,以小鼠前额叶皮质为目标脑区,考察XBXT-2对皮质场电位的影响进而探索其药理作用机制。方法在C57BL/6J小鼠颅顶手术埋置皮质电极(ECOG),恢复7 d后灌胃给予XBXT-2100 mg/kg,1 h后记录前额叶皮质局部场电位(LFP)30 min。数据采用Neuro Explorer软件进行频谱分析,给药前后α、β、θ、γ和δ等脑电频段的功率谱密度变化采用GraphPad Prism 10.3进行分析,θ和γ振荡的相位-振幅耦合采用Matlab 2021软件分析。结果与空白对照组比较,灌胃给予XBXT-2后,小鼠前额叶皮质高频γ振荡受到抑制,且θ、β、α和δ振荡均升高,同时增强θ和γ振荡的相位振幅耦合。结论XBXT-2可能通过调节小鼠前额叶皮质神经网络活动、增强θ和γ振荡的相位振幅耦合发挥情绪和记忆调节作用。
Objective To investigate the pharmacological mechanism through which total flavonoids extracted from Xiaobuxin⁃Tang(XBXT⁃2)affects neural network activities in the frontal cortex by focusing on the effects of XBXT⁃2 on the cortical field potentials in the frontal association cortex(FrA)in mice.Methods Cortical electrodes were implanted into the skull of C57BL/6J mice targeting the FrA.After a 7⁃day recovery period,the mice were administered XBXT⁃2 intragastrically at a dose of 100 mg/kg,and 1 hour later,local field potential(LFP)in the FrA were recorded for 30 minutes.Spectral analysis of the data was performed using Neuro Explorer software.Changes in the power spectral density ofα,β,θ,γ,andδfrequency bands before and after drug administration were analyzed using GraphPad Prism 10.3.Phase⁃amplitude coupling ofθandγoscillations was analyzed using Matlab 2021 software.Results It was found that the oral administration of XBXT⁃2 significantly suppressed high⁃frequencyγoscillations while simultaneously enhancingθ,β,α,andδoscillations in FrA of mice compared to the control.Furthermore,XBXT⁃2 treatment markedly strengthened the phase⁃amplitude coupling betweenθandγoscillations.Conclusion XBXT⁃2 possibly affects emotional and cognitive functions by modulating neural network activity in FrA and enhancingθ⁃γphase⁃amplitude coupling in mice.
作者
杨进
李劲草
张树卓
王彦凯
邱仁洪
顾永芳
薛瑞
李硕
张杨
范琼尹
张有志
刘爽
YANG Jin;LI Jincao;ZHANG Shuzhuo;WANG Yankai;QIU Renhong;GU Yongfang;XUE Rui;LI Shuo;ZHANG Yang;FAN Qiongyin;ZHANG Youzhi;LIU Shuang(Basic Medical College,Jiamusi University,Jiamusi,Heilongjiang 154007,China;Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《军事医学》
2025年第10期747-753,共7页
Military Medical Sciences
基金
北京市自然科学基金项目(7254511)
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023311)。
