摘要
目的探讨急性高原不同暴露时间对小鼠食欲和肠道菌群的影响。方法随机将30只7~8 w龄SPF级C57BL/6J雄性小鼠分为3组,每组10只,设置为平原对照组(P组)、急性高原1 d组(G1组)、急性高原7 d组(G7组)。其中,P组始终处于动物房喂养;G7组在实验开始后立即转移至高原环境模拟舱中(模拟海拔5000 m)直至实验结束;G1组则在动物房喂养3 d后转移至高原环境模拟舱中(模拟海拔5000 m)1 d。实验过程中每日称量小鼠体重和饲料质量并记录;环境模拟结束时,使用冻存管收集小鼠粪便,摘除眼球采血法采集小鼠血液并分离血清。使用ELISA试剂盒测定小鼠血清神经肽Y(neuropeptide Y,NPY)、酪酪肽(peptides YY,PYY)等食欲激素含量。小鼠粪便样本进行DNA抽提后,选择16S rRNA可变区中的V3-V4区域进行PCR扩增,并进行DNA测序分析和功能分类注释。结果急进高原后,小鼠血清PYY含量升高、NPY含量下降;其中,G1组与P组差异性有统计学意义(P<0.05)。急进高原后的低压缺氧环境影响小鼠的肠道菌群多样性构成,双歧杆菌属(Bifidobacterium)等益生菌的相对丰度下降,肠球菌属(Enterococcus)等菌属相对丰度上升;COG基因功能注释结果显示小鼠肠道菌群中碳水化合物、氨基酸以及能量代谢对应的功能特征丰度均较高。结论急性高原暴露对小鼠食欲有明显抑制作用,且对小鼠肠道菌群多样性构成产生影响。
Objective To explore the effect of different acute high-altitude exposure durations on appetite and intestinal flora in mice.Methods Thiry SPF C57BL/6J male mice aged 7-8 weeks were randomly divided into 3 groups,with 10 mice in each group:plain control group(group P),acute plateaus 1 day group(group G1),and acute plateaus 7 days group(group G7).Among them,Group G7 was transferred to the plateau environment simulation cabin(simulated altitude of 5000 m)immediately after the experiment began until the experiment ended.Group P was fed in the plain animal house.Group G1 was fed in the plain animal house for 3 days,and then transferred to the simulated cabin of plateau environment(simulated altitude of 5000 m)for 1 day.During the experiment,the body weight and food intake were recorded every day.At the end of the environmental simulation,feces samples were collected into freezing tube,and blood samples were collected from orbital vein and serum was separated.The contents of NPY and PYY in the serum were determined by ELISA kits.After DNA extraction from mouse feces samples,the V3-V4 region in the variable region of 16S rDNA was selected for PCR amplification,DNA sequencing analysis and functional classification annotation.Results The acute plateaus exposure obviously inhibited the appetite of mice,and at the same time caused the weight loss.After entering the plateau,serum content of PYY increased and the NPY decreased.Among them,there was a significant difference between the group G1 and the group P(P<0.05).The low-pressure hypoxia exposure affected the composition of intestinal flora diversity in mice.The relative abundance of probiotics such as Bifidobacterium decreased,while the relative abundance of Enterococcus increased.The functional annotation results of COG gene showed that the functional characteristics of carbohydrates,amino acids and energy metabolism in the intestinal flora were increased.Rapid entry into the plateau environment adversely affected the generation and transformation of energy in intestinal flora and the expression of related genes such as biosynthesis,transport and catabolism of secondary metabolites.Conclusion Acute high-altitude exposure significantly suppresses the appetite and affected the composition of intestinal flora diversity.
作者
陈潇楠
李晓莉
吴生财
吴刚
刘宝
梁宪帅
CHEN Xiao-nan;LI Xiao-li;WU Sheng-cai;WU Gang;LIU Bao;LIANG Xian-shuai(Air Force Logistics Academy,Xuzhou 221000,China;Army Logistics Academy,Chongqing 401331,China;Army Medical University,Chongqing 400038,China)
出处
《营养学报》
北大核心
2025年第4期383-390,共8页
Acta Nutrimenta Sinica
关键词
急性高原
小鼠
食欲
肠道菌群
神经肽Y
酪酪肽
acute plateaus
mice
appetite
intestinal flora
neuropeptide Y
peptides YY
