摘要
糖基化是一种非常重要的蛋白质翻译后修饰,人体内超过50%的蛋白具有糖基化修饰,能够影响蛋白质的生物学功能参与多种生物学过程。在癌症、炎症或代谢性疾病的发生与进展等多种生理病理过程中会发生糖基化修饰的异常,因此,糖基化修饰的检测至关重要。经典的糖基化修饰检测方法,如流式细胞术、质谱等方法对于痕量的糖基化信号难以检测。该文开发了一种基于DNA扩增的糖链检测方法,将识别糖基化修饰的凝集素偶联单链DNA(ssDNA),凝集素与糖链的识别与结合把“糖信号”转化为“核酸信号”,以ssDNA为模板进行PCR级联放大,能够实现痕量的糖基化信号的检测,为糖生物学研究和临床糖组学标志物发现提供了新的视角。
Glycosylation is an essential post-translational modification.Over 50%of proteins in the human body undergo glycosylation,which can influence their biological functions and participate in various biological processes.Abnormal glycosylation occurs in a range of physiological and pathological processes,such as cancer,inflammation,or metabolic diseases,making the detection of glycosylation essential.Classical methods for detecting glycosylation,like flow cytometry and mass spectrometry,often struggle with trace-level glycosylation signals.This study reports a DNA amplification-based method for glycan detection.Lectins that recognize and bind certain glycosylations are labeled with single-stranded DNA(ssDNA).The specific binding between lectins and glycans convert the"glycan signal"into a"nucleic acid signal".Using the ssDNA as a template for PCR amplification enables the detection of trace glycosylation signals,offering a new perspective for glycobiology research and the discovery of clinical glycomarker.
作者
谭增琦
张慧娜
曹琳
TAN Zengqi;ZHANG Huina;CAO Lin(Shaanxi Key Laboratory of Biotechnology and Biochemical Engineering,School of Medicine,Northwest University,Xi'an 710069,China;Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education/College of Life Science,Northwest University,Xi'an 710069,China)
出处
《西北大学学报(自然科学版)》
北大核心
2025年第6期1364-1374,共11页
Journal of Northwest University(Natural Science Edition)
基金
国家自然科学基金(32271338)。
