摘要
对一株自主分离保藏的链霉菌HBERC-20716进行了鉴定,并评价了其对棉花枯萎病的防治效果及其对棉花苗期光合作用与促生作用的影响。经形态学与16S rRNA基因序列分析,将该菌株鉴定为浅多色链霉菌(Streptomyces pluricolorescens)。室内穴盘试验结果表明,链霉菌HBERC-20716对室内穴盘盆栽试验棉花枯萎病具有显著的抑制作用,植株倒苗率显著低于对照处理。链霉菌HBERC-20716菌株发酵液处理室内棉花幼苗试验结果表明,链霉菌HBERC-20716菌株发酵液对棉花具有促生作用,棉花幼苗株高、植株鲜重、根长、根重均显著高于对照;光合作用测定表明,链霉菌HBERC-20716能促进棉花光合作用,净光合速率、蒸腾速率、气孔导度均得以显著提高,同时大幅降低了细胞间CO_(2)浓度。
A strain of Streptomyces sp.HBERC-20716,which was independently isolated and preserved,was identified,and its efficacy against cotton fusarium wilt as well as its effects on photosynthesis and growth promotion at the cotton seedling stage were evaluated.Through morphological and 16S rRNA gene sequence analyses,the strain was identified as Streptomyces pluricolorescens.Results from indoor pot experiments demonstrated that Streptomyces pluricolorescens HBERC-20716 significantly inhibited the occurrence of cotton fusarium wilt,with a markedly lower seedling blight rate compared to the control.Treatment of cotton seedlings with the fermentation broth of Streptomyces pluricolorescens HBERC-20716 indicated its growth-promoting effect on cotton,as evidenced by plant height,fresh weight,root length,and root weight of the seedlings being significantly greater than those in the control.Measurements of photosynthesis parameters showed that Streptomyces pluricolorescens HBERC-20716 promoted photosynthetic performance,significantly increasing the net photosynthetic rate,transpiration rate,and stomatal conductance,while substantially reducing the intercellular CO_(2) concentration.
作者
龙同
万中义
王孝刚
张友昌
艾伦强
冯常辉
LONG Tong;WAN Zhong-yi;WANG Xiao-gang;ZHANG You-chang;AI Lun-qiang;FENG Chang-hui(Key Laboratory of Biology and Genetic Breeding of Cotton in the Middle Reaches of the Yangtze River,Ministry of Agriculture and Rural Affairs,Wuhan 430064,Hubei;Hubei Biopesticide Engineering Research Center,Wuhan 430064,China;Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China)
出处
《湖北农业科学》
2025年第12期99-103,116,共6页
Hubei Agricultural Sciences
基金
湖北省棉花科技支撑项目(K20250814076)
武汉市科技特派员产学研专项(2023110201030680)
农业农村部长江中游棉花生物学与遗传育种重点实验室开放课题(CBB2022A01)
湖北省自然科学联合基金项目(2024AFD148)
恩施州科技支撑项目(D20230080)
“农业农村部中药材生物学与栽培重点实验室(武汉)”开放课题(BZ2024004)
湖北省农业科技创新中心创新团队项目(2021-620-000-001-027)。
