摘要
农田土壤中的雌二醇(E2)不仅污染土壤环境,还会转移至水体和农作物中,进而危害人体健康。微生物修复是环境中E2原位修复的重要方式,但目前E2的修复菌株多分离于水体环境,与农田土壤E2微生物修复技术开发的适配性还较低。在长期施用畜禽粪便的农田土壤中成功分离和富集得到8株能够有效降解E2的菌株,分别属于寡养单胞菌属(Stenotrophomonas sp.)、鞘氨醇杆菌属(Sphingobacterium sp.)、芽孢杆菌属(Bacillus sp.),其中寡养单胞菌属当属E2降解的优势菌属。特别是,寡养单胞菌属的OE2-1降解效率最高,该菌株对温度、pH、底物浓度适应能力较强,在初始温度30~40℃、初始pH5~9、底物质量浓度1~10 mg/L较宽的环境条件下都能保持7 d 80%以上的E2降解率,与已报道的E2降解菌能力相当,可为农田土壤中微生物降解E2提供菌种资源,从而为E2污染土壤修复提供技术支撑。
The 17β-estradiol(E2)in farmland soil not only pollutes the soil environment,but also transfers to water bodies and agricultural crops,thereby endangering human health.Microbial remediation is an important method for in-situ remediation of E2 in the environment.However,most of the E2 degrading strains are currently derived from water environment,which exhibits relatively low compatibility with the development of E2 microbial remediation technologies in farmland soils.8 effective E2 degrading bacteria were isolated and concentrated from farmland soil treated with animal manure compost for a long time.These strains belonged to Stenotrophomonas sp.,Sphingobacterium sp.and Bacillus sp.,among which Stenotrophomonas sp.was the dominant species for E2 degrading.Especially,Stenotrophomonas sp.OE2-1 had the highest degradation efficiency.This strain exhibited excellent adaptability to initial temperature,initial pH and substrate concentration.It maintained high degradation rate(over 80%)of E2 within 7 days under a wide environmental conditions of initial temperature 30-40℃,initial pH5-9,and substrate mass concentration 1-10 mg/L.The degradation efficiency of this strain was comparable to that of known E2 degrading bacteria before.This study could provide microbial resources for the degradation of E2 in farmland soil,and further provide effective support for E2 pollution soil remediation.
作者
宋胜霞
杨硕
司佳艺
李帅佳
孙玉婷
韩卉
SONG Shengxia;YANG Shuo;SI Jiayi;LI Shuaijia;SUN Yuting;HAN Hui(College of Life Sciences,Langfang Normal University,Langfang Hebei 065000;School of Ocean and Environment,Tianjin University of Science and Technology,Tianjin 300457;Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province,Langfang Hebei 065000)
出处
《环境污染与防治》
北大核心
2025年第12期44-50,I0003,共8页
Environmental Pollution & Control
基金
河北省自然科学基金资助项目(No.D2024408001)
河北省高等学校科学技术研究项目(No.QN2023254)。
关键词
雌二醇
农田土壤
降解条件
降解能力
微生物修复
17β-estradiol
farmland soil
degradation conditions
degradation efficiency
microbial remediation
