摘要
目的建立在线凝胶渗透色谱-气相色谱-串联质谱法(gel permeation chromatography-gas chromatography-tandem mass spectrometry,GPC-GC-MS/MS)快速检测食用油样品中17种有机紫外吸收剂(organic ultraviolet absorbers,OUVs)的方法。方法采用在线GPC技术与GC-MS/MS相结合,高效去除食用油中的脂质。目标化合物使用DB-5MS(30 m×0.25 mm,0.25µm)分离,多反应监测(multiple reaction monitoring,MRM)模式下扫描,同位素内标法定量。结果0.2~100.0 ng/mL范围内,17种OUVs的线性关系良好,相关系数均在0.9970以上,检出限为0.3~0.6 ng/g。在2、10、50 ng/g 3个不同浓度水平下进行加标回收试验,17种OUVs的平均加标回收率为80.0%~117.6%,相对标准偏差(relative standard deviations,RSDs)为1.3%~13.7%。采用本方法对食用油样品进行检测,发现多种OUVs单体均有不同浓度检出。结论本方法操作简单、高效便捷、灵敏度高,适合食用油样品中17种OUVs的快速测定。
Objective To establish a rapid method for determination of 17 kinds of organic ultraviolet absorbers(OUVs)in edible oil samples by online gel permeation chromatography-gas chromatography-tandem mass spectrometry(GPC-GC-MS/MS).Methods Online GPC technology was combined with GC-MS/MS to efficiently remove lipids from edible oils.Target compounds were separated using a DB-5MS column(30 m×0.25 mm,0.25μm)and scanned in multiple reaction monitoring(MRM)mode,with quantification performed by the isotope internal standard method.Results Good linearity was observed for all 17 kinds of OUVs within the range of 0.2-100.0 ng/mL,with correlation coefficients above 0.9970.The limits of detection ranged from 0.3 to 0.6 ng/g.The average spiked recovery rates of 17 kinds of OUVs at 3 concentration levels(2,10 and 50 ng/g)ranged between 80.0%and 117.6%,with relative standard deviations(RSDs)of 1.3%to 13.7%.The edible oil samples were tested using this method,and it was found that a variety of OUVs monomers were detected at different concentrations.Conclusion This method is simple,efficient,convenient and highly sensitive,making it suitable for determination 17 kinds of OUVs in edible oil samples.
作者
刘潇
杨梦
王旭博
周妍
曹文成
陈海川
程青云
闻胜
LIU Xiao;YANG Meng;WANG Xu-Bo;ZHOU Yan;CAO Wen-Cheng;CHEN Hai-Chuan;CHENG Qing-Yun;WEN Sheng(Hubei Provincial Center for Disease Control and Prevention,National Health Commission Specialty Laboratory of Food Safety Risk Assessment and Standard Development,Wuhan 430079,China;Xiangyang Ecological Environmental Monitoring Center,Department of Ecology and Environment of Hubei Province,Xiangyang 441000,China)
出处
《食品安全质量检测学报》
2025年第24期218-225,共8页
Journal of Food Safety and Quality
基金
国家重点研发计划项目(2023YFF1104800)
湖北省自然科学基金项目(2022CFB022)
楚天英才项目(S2024JY04)。
关键词
食用油
有机紫外吸收剂
在线凝胶渗透色谱法
气相色谱-串联质谱法
edible oil
organic ultraviolet absorbers
online gel permeation chromatography
gas chromatography-tandem mass spectrometry
