摘要
目的根据全基因组测序分析3株水产品来源的霍乱弧菌分子分型、毒力基因和耐药基因等特征。方法对2024年采集自邯郸市农贸市场和流通环节的鱼类、虾类和双壳类等海产品共90份水产样品,进行霍乱弧菌的检测,对分离菌株进行药敏实验、毒力基因检测和全基因组测序分析。结果90份样品中共检出3株霍乱弧菌,均为非O1/O139群,所有分离株均不携带ctxAB基因,3株菌的基因组序列长度分别为585704、872425和353640 bp,GC含量平均为47.54%,基因覆盖度平均为87.72%,通过pubMLST比对分子分型分别为ST1787型、ST203型、ST518型。3个分离株分别包含365、371和383个毒力相关基因,携带耐药基因的数量分别为353、362和365个。结论通过全基因组测序了解邯郸市2024年水产品中霍乱弧菌的生化特性以及优势菌株,为食品安全风险评估、全市霍乱防控提供数据支持。
Objective To understand the characteristics such as molecular typing,virulence genes and drug resistance genes of 3 Vibrio cholerae strains from aquatic products based on whole genome sequencing.Methods A total of 90 aquatic product samples such as fish,shrimp and bishells collected from farmers’markets and circulation links in Handan City in 2024 were tested for Vibrio cholerae.Drug sensitivity tests,virulence gene tests and whole genome sequencing analyses were conducted on the isolated strains.Results A total of 3 Vibrio cholerae strains were detected in 90 samples,all of which belonged to the non-O1/O139 group.None of the isolates carried the ctxAB gene.The genomic sequence lengths of the 3 strains were 585704,872425 and 353640 bp respectively,and the average GC content was 47.54%.The average gene coverage was 87.72%.Through pubMLST comparison,the molecular typing was ST1787 type,ST203 type and ST518 type respectively.The three isolates contained 365,371,and 383 virulence-associated genes,respectively,and carried 353,362,and 365 resistance genes.Conclusion Through whole-genome sequencing to understand the biochemical characteristics and dominant strains of Vibrio cholerae in Handan City in 2024,it provides data support for food safety risk assessment and cholera prevention and control throughout the city.
作者
闫永飞
陈彩霞
张璐
白文轩
雒月云
李瑞芳
YAN Yong-Fei;CHEN Cai-Xia;ZHANG Lu;BAI Wen-Xuan;LUO Yue-Yun;LI Rui-Fang(Handan Center for Disease Control and Prevention,Handan 056008,China)
出处
《食品安全质量检测学报》
2025年第24期192-198,共7页
Journal of Food Safety and Quality
基金
邯郸市科技计划项目(23422083171)。
关键词
霍乱弧菌
全基因组测序
毒力基因
耐药基因
Vibrio cholerae
whole genome sequencing
virulence genes
drug resistance gene
