摘要
真菌污染是粮食安全的重要威胁之一,为有效检测和防控有害真菌,确保粮食安全和质量,本研究对浙江省宁波地区4个品种稻谷(甬优15、甬优538、甬优1540和甬优7860)的真菌群落进行分析。采用高通量测序技术分析真菌群落多样性,结果显示4个品种稻谷中共有OTU为326种,分属4门,18纲,41目,77科,117属,151种,其中子囊菌门丰度最高,占比96.6%。α多样性指数分析表明样品真菌群落具有较好的丰富度、多样性和均匀性。通过纯培养技术分离纯化获得203株真菌,结合形态学特征观察和分子生物学鉴定,发现优势真菌主要分布在曲霉属、镰刀菌属、青霉属和帚枝霉属。对优势菌株进行分子生物学鉴定,确定主要优势种为黄曲霉和禾谷镰刀菌。研究结果可为稻谷真菌病害的预防控制和南方地区科学储粮策略的制定提供理论参考。
Fungal contamination represents a significant threat to food security.To effectively detect and control harmful fungi and ensure food safety and quality,this study analyzed the fungal communities in four varieties of Yongyou rice(Yongyou 15,Yongyou 538,Yongyou 1540,and Yongyou 7860)from Ningbo,Zhejiang Province.High-throughput sequencing analysis of fungal community diversity revealed 59 shared OTUs among the four rice varieties,belonging to 4 phyla,18 classes,41 orders,77 families,117 genera,and 151 species,with Ascomycota showing the highest abundance at 96.6%.Alpha diversity index analysis indicated that the fungal communities in the samples exhibited good rich ness,diversity,and evenness.Through pure culture techniques,203 fungal strains were isolated and purified.Based on morphological characteristics observation and molecular biological identification,the dominant fungi were mainly distributed in Aspergillus,Fusarium,Penicillium,and Sarocladium.Further molecular identification of dominant strains confirmed the main dominant species as Aspergillus flavus and Fusarium graminearum.The findings provide theoretical basis for the prevention and control of fungal diseases in rice and the development of scientific grain storage strategies in southern regions.
作者
刘悦
王馨怡
陈钱峰
张慧恩
沈存宽
杨华
LIU Yue;WANG Xinyi;CHEN Qianfeng;ZHANG Huien;SHEN Cunkuan;YANG Hua(Zhejiang Wanli University,Ningbo 315100,Zhejiang)
出处
《中国食品学报》
北大核心
2025年第11期364-372,共9页
Journal of Chinese Institute of Food Science and Technology
基金
浙江省一流学科“生物工程”学生创新项目(CX2024023,CX202434)。
关键词
稻谷
真菌群落
高通量测序
分子鉴定
形态特征
rice
fungal community
high-throughput sequencing
molecular identification
morphological characteristics
