摘要
为了探索褪黑素对微藻生物量以及代谢产物的影响,以莱茵衣藻为研究对象,使用不同浓度褪黑素(0,5,20μmol/L)对该藻进行处理,通过生物量筛选最佳的褪黑素处理浓度。结果表明,通过真空干燥法将收集的微藻细胞烘干至恒重,发现5μmol/L褪黑素处理的微藻干质量在第7天达到0.443 g/L,显著高于未添加褪黑素处理的微藻细胞。通过氯仿/甲醇提取法对收集的微藻细胞脂质含量进行测定,5μmol/L褪黑素处理组的最高脂质含量达到微藻干重的40.3%,与对照组相比显著提高了19.2%。同时褪黑素处理的莱茵衣藻中其它的初级代谢产物(蛋白质和多糖)以及次级代谢产物(色素、总酚、总黄酮、抗坏血酸和谷胱甘肽)的含量显著提高。外源褪黑素处理可通过提高莱茵衣藻中色素含量,提高莱茵衣藻的光合作用能力,最终提高莱茵衣藻生物量,同时诱导高价值代谢产物积累。研究结果为新型食品莱茵衣藻的工业化生产提供新的思路。
In order to explore the effect of melatonin on the biomass as well as metabolites of microalgae,Chlamydomonas reinhardtii was treated with different concentrations of melatonin(0,5,20μmol/L)to screen the optimal melatonin treatment concentration by biomass.The results showed that by vacuum drying method,the collected microalgae cells were dried to constant weight and the dry weight of 5μmol/L melatonin-treated microalgae was found to reach 0.443 g/L on day 7,which was significantly higher than that of the microalgae cells without melatonin.The lipid content of the collected microalgae cells was determined by the chloroform/methanol extraction method,and it was found that the highest lipid content in the 5μmol/L melatonin treatment group reached 40.3%of the dry weight of microalgae,which was significantly increased by 19.2%compared to the control group,and the contents of other primary metabolites(proteins and polysaccharides)as well as secondary metabolites(pigments,total phenolic,total flavonoid,ascorbic acid,and glutathione)of Chlamydomonas reinhardtii were also significantly increased by melatonin treatment.Exogenous melatonin could increase the photosynthetic capacity of Chlamydomonas reinhardtii by increasing the pigment content,thereby increasing the biomass and inducing the accumulation of high-value metabolites at the same time in Chlamydomonas reinhardtii.This study provided a new idea for the industrial production of Chlamydomonas reinhardtii as a novel food.
作者
刘浩楠
邢晓凡
刘长虹
LIU Haonan;XING Xiaofan;LIU Changhong(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601)
出处
《中国食品学报》
北大核心
2025年第11期265-273,共9页
Journal of Chinese Institute of Food Science and Technology
基金
国家自然科学基金项目(U23A2081)
中央高校基本科研业务费项目(JZ2024HGTG0286)。
关键词
褪黑素
莱茵衣藻
初级代谢产物
次级代谢产物
melatonin
Chlamydomonas reinhardtii
primary metabolites
secondary metabolites
