摘要
目的:探讨米诺地尔酊基于Wnt/β-连环蛋白(β-catenin)通路治疗雄激素性脱发(AGA)的药效及作用机制。方法:小鼠随机分为对照组、AGA组、米诺地尔酊组、DKK-1(Wnt/β-catenin通路抑制剂)组、米诺地尔酊+DKK-1组,每组12只。除对照组外,其他组小鼠均通过连续14 d每天向脱毛区涂抹1次0.1 ml 8%丙酸睾酮诱导AGA模型。建模第1天各组开始给药处理,1次/天,持续60 d。检测小鼠脱毛区毛发覆盖率、毛发重量、毛发长度;HE染色观察小鼠脱毛区皮肤组织中毛囊状态;qRT-PCR检测小鼠脱毛区皮肤组织中胰岛素样生长因子(IGF-1)、血管内皮生长因子(VEGF)mRNA表达;ELISA检测小鼠脱毛区皮肤组织中双氢睾酮、睾酮、活性氧(ROS)、丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性;Western blot检测小鼠脱毛区皮肤组织中Wnt3a、β-catenin蛋白。结果:与对照组相比,AGA组小鼠皮肤组织中毛囊大多数位于表皮,毛囊数量少,脱毛区毛发覆盖率、毛发重量、毛发长度降低,脱毛区皮肤组织中双氢睾酮、睾酮水平、ROS、MDA水平升高,脱毛区皮肤组织中IGF-1、VEGF mRNA表达、SOD活性及Wnt3a、β-catenin蛋白降低(P<0.05);与AGA组相比,米诺地尔酊组小鼠毛囊均位于真皮层,毛囊数量增多,脱毛区毛发覆盖率、毛发重量、毛发长度降低,脱毛区皮肤组织中双氢睾酮、睾酮水平、ROS、MDA水平降低,脱毛区皮肤组织中IGF-1、VEGF mRNA表达、SOD活性及Wnt3a、β-catenin蛋白升高,DKK-1组小鼠上述指标变化与米诺地尔酊组相反(P<0.05);DKK-1逆转了米诺地尔酊对AGA小鼠雄激素水平及氧化应激的影响。结论:米诺地尔酊可能通过激活Wnt/β-catenin通路降低雄激素水平,抑制氧化应激,进而改善小鼠AGA。
Objective To investigate the efficacy and mechanism of minoxidil tincture in treating Androgenetic Alopecia(AGA)based on the Wnt/β-catenin pathway.Methods Mice were randomly assigned into a control group,AGA group,minoxidil tincture group,DKK-1(Wnt/β-catenin pathway inhibitor)group,and minoxidil tincture+DKK-1 group,with 12 mice in each group.Except for the control group,mice in all other groups induced AGA model by applying 0.1 ml of 8%testosterone propionate once a day to the depilation area for 14 consecutive days.On the first day of modeling,each group began to receive medication treatment once a day for 60 days.The hair coverage,hair weight,and hair length in the depilation area of mice were detected.HE staining was applied to observe the status of hair follicles in the depilated skin tissue of mice.QRT-PCR was applied to detect the mRNA expression of Insulin-like Growth Factor(IGF-1)and Vascular Endothelial Growth Factor(VEGF)in the skin tissue of depilated mice.ELISA was applied to detect the levels of dihydrotestosterone,testosterone,Reactive Oxygen Species(ROS),Malondialdehyde(MDA),and Superoxide Dismutase(SOD)activity in the skin tissue of depilated mice.Western blot was applied to detect Wnt3a andβ-catenin proteins in the depilated skin tissue of mice.Results Compared with the control group,the majority of hair follicles in the skin tissue of mice in AGA group were located in the epidermis,with fewer follicles,the hair coverage,weight,and length in the depilated area were reduced,the levels of dihydrotestosterone,testosterone,ROS,and MDA in the skin tissue of the hair removal area were increased,the expression of IGF-1 and VEGF mRNA,SOD activity,and Wnt3a andβ-catenin proteins in the skin tissue of the depilation area were reduced(P<0.05).Compared with the AGA group,the mice in the minoxidil tincture group had hair follicles located in the dermis layer,the number of hair follicles increased,and the hair coverage,weight,and length in the hair removal area decreased,the levels of dihydrotestosterone,testosterone,ROS,and MDA in the skin tissue of the hair removal area were reduced,the expression of IGF-1 and VEGF mRNA,SOD activity,and Wnt3a andβ-catenin proteins in the skin tissue of the depilation area were elevated,the changes in the above indicators in the DKK-1 group were opposite to those in the minoxidil tincture group(P<0.05).DKK-1 reversed the efects of minoxidil tincture on androgen levels and oxidative stress in AGA mice.Conclusion Minoxidil tincture may reduce androgen levels,inhibit oxidative stress,and improve mouse AGA by activating the Wnt/β-catenin pathway.
作者
吴美超
姜鹏爽
高晓冉
刘翊
刘冰
石云
WU Meichao;JIANG Pengshuang;GAO Xiaoran;LIU Yi;LIU Bing;SHI Yun(Department of Dermatology,the Second Affiliated Hospital of Xingtai Medical College,Xingtai 054000,Hebei,China;Department of Traditional Chinese Medicine,the Second Affiliated Hospital of Xingtai Medical College,Xingtai 054000,Hebei,China)
出处
《中国美容医学》
2026年第1期9-13,共5页
Chinese Journal of Aesthetic Medicine
基金
河北省中医药管理局科研计划项目(编号:2022645)。
