摘要
目的:旨在通过生物信息学技术探索与皮肤烧伤相关的关键基因。方法:从GSE8056数据库获取烧伤芯片矩阵,通过R语言分析获取差异基因并同时使用韦恩图与糖酵基因取交集获得交集基因,并进行GO和KEGG富集分析;使用LASSO回归和随机森林算法鉴定关键基因,将GSE77791作为外部验证集进行验证,CIBERSORT算法用于免疫浸润分析,最后利用Connectivity Map数据库对差异基因进行药物靶向预测。结果:从GSE8056获得1355个差异基因,取交集得到100个差异基因,GO分析主要涉及正调控血管生成,正调控血管发育,上皮细胞增殖等生物过程,KEGG分析显示HIF-1信号通路,IL-17信号通路,细胞周期信号通路与烧伤过程密切相关。机器学习方法筛选出PNP、DENND4C、FBXW7、THBS1这4个关键基因。免疫浸润结果显示烧伤组织中静息NK细胞,M0巨噬细胞,活化的肥大细胞,中性粒细胞水平显著高于正常组织,并且通过相关性分析发现关键基因与免疫过程具有显著相关性。最后通过分析得出Lestaurtinib、PI-828、PIK-90、Selumetinib是有治疗烧伤潜力的药物。结论:PNP、DENND4C、FBXW7、THBS1是皮肤烧伤发展过程中的关键基因,并与免疫浸润显著相关,为未来研究烧伤发展机制和药物研究提供了基础。
Objective The aim of this study was to explore the key genes associated with skin burn through bioinformatics.Methods The burn gene microarray was obtained from GSE8056 database,the diferential genes were obtained by R language analysis,Venn diagram was used to obtain the intersection of diferential genes and glycolysis related genes,GO and KEGG enrichment analysis were then performed.LASSO regression and random forest algorithm were used to identify key genes,GSE77791 was used as an external validation set.CIBERSORT algorithm was used for immune infiltration analysis,and drugs targeting diferential genes were predicted by Connectivity Map database.Results 1355 diferential genes were obtained from GSE8056,and 100 diferential genes were obtained by intersection analysis.GO analysis mainly involved positive regulation of angiogenesis,vascular development,epithelial cell proliferation and so on.KEGG analysis showed that HIF-1 signaling pathway,IL-17 signaling pathway and cell cycle signaling pathway were closely related to burn process.Four key genes,PNP,DENND4C,FBXW7 and THBS1,were screened by machine learning method.Immune infiltration results showed that resting NK cells,M0 macrophages,activated mast cells and neutrophils in burn tissues were significantly higher than those in normal tissues,and correlation analysis showed that key genes were significantly correlated with immune processes.Lestaurtinib,PI-828,PIK-90 and Selumetinib were potential drugs for the treatment of burns.Conclusion PNP,DENND4C,FBXW7 and THBS1 are the key genes in the development of skin burn and significantly related to immune infiltration,the result provides the foundation for our future study on burn injury and drug research.
作者
徐双依
徐刚
XU Shuangyi;XU Gang(Department of Plastic and Burn Surgery,Northern Jiangsu People's Hospital Affiliated to Yangzhou University/Northern Jiangsu People's Hospital,Yangzhou 225009,Jiangsu,China;Graduate School of Yangzhou University,Yangzhou 225009,Jiangsu,China)
出处
《中国美容医学》
2026年第1期1-8,共8页
Chinese Journal of Aesthetic Medicine
