摘要
该研究以竹叶黄酮提取物为原料,分别添加单金属离子Al^(3+)、Fe^(3+)、Cu^(2+)、Na^(+)、K^(+)、Ca^(2+)与竹叶黄酮在乙醇溶液中相互反应,对比金属离子-竹叶黄酮复合醇溶液的颜色参数及其在高温条件下的稳定性,随后利用电子顺磁共振波谱仪技术检测竹叶黄酮醇溶液和Al^(3+)、Fe^(3+)等金属离子-竹叶黄酮复合醇溶液在磁场中自由基的强度。结果表明,Al^(3+)、Fe^(3+)能与竹叶黄酮快速络合,并导致竹叶黄酮醇溶液发生极显著的颜色变化(P<0.01);Cu^(2+)只在高温条件下与竹叶黄酮络合并导致显著的颜色变化(P<0.05);Na^(+)、K^(+)、Ca^(2+)基本不与竹叶黄酮络合且不会发生颜色变化。Al^(3+)因增强竹叶黄酮的电子分布而增强对DPPH·、O2-·的清除能力,但减弱对OH·的清除能力;Fe^(3+)因其氧化活性和对竹叶黄酮结构的破坏,同步减弱对DPPH·、O2-·和OH·的清除能力。因此,确定Al^(3+)、Fe^(3+)等金属离子因影响机制不同,会对竹叶黄酮醇溶液的色差和抗氧化活性产生不同的影响。
Using bamboo leaf flavone extract as raw material,single metal ions Al^(3+),Fe^(3+),Cu^(2+),Na^(+),K^(+),Ca^(2+)were added to react with bamboo leaf flavonoids in ethanol solution,respectively.The color parameter and stability of the metal ion bamboo leaf flavone composite alcohol solution under high temperature conditions were determined.Then the intensity of free radicals in bamboo leaf flavone solution and metal ion(such as Al^(3+),Fe^(3+))bamboo leaf flavone composite alcohol solution was detected and compared by electronic paramagnetic resonance spectroscopy.The results showed that Al^(3+)and Fe^(3+)could rapidly complex with bamboo leaf flavonoids and cause extremely significant color changes in the bamboo leaf flavonoid alcohol solution(P<0.01);Cu^(2+)only complexed with bamboo leaf flavonoids under high temperature conditions,resulting in significant color changes(P<0.05).Na^(+),K^(+),Ca^(2+)did not complex with bamboo leaf flavonoids and did not undergo color changes.Al^(3+)enhanced the electron distribution of bamboo leaf flavonoids and improved their ability to remove DPPH·and O2-·,but weakened their ability to remove OH·.Fe^(3+)simultaneously weakened its ability to scavenge DPPH·,O2-·and OH·,due to its oxidative activity and damage to the structure of bamboo leaf flavonoids.Therefore,it was determined that metal ions Al^(3+)and Fe^(3+)had different effect on the color difference and antioxidant activity of bamboo leaf flavonoids due to their different influencing mechanisms.
作者
易翔
黄天琪
陈科宇
王利
施鹏
罗高建
童国强
杨强
YI Xiang;HUANG Tianqi;CHEN Keyu;WANG Li;SHI Peng;LUO Gaojian;TONG Guoqiang;YANG Qiang(Jing Brand Co.,Ltd.,Huangshi 435100,China;School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Jinpai Moutai Town Liquor Co.,Ltd.,Zunyi 564500,China)
出处
《中国酿造》
北大核心
2025年第12期171-175,共5页
China Brewing
基金
湖北省重点研发计划(2023BBB004)。
关键词
金属离子
竹叶黄酮
颜色参数
抗氧化活性
metal ion
bamboo flavonoids
color parameter
antioxidant activity
