摘要
【目的】通过测定大豆食心虫Leguminivora glycinivorella不同地理种群老熟幼虫呼吸代谢关键酶活性对温度的响应,明确该虫不同地理种群对温度变化的适应性。【方法】利用生化方法测定温度18,21,24,27和30℃下大豆食心虫4个地理种群(广西都安、贵州合肥、安徽宿州和吉林长春种群)老熟幼虫呼吸代谢关键酶3-磷酸甘油醛脱氢酶(3-phosphoglyceraldehyde dehydrogenase,GAPDH)、3-磷酸甘油脱氢酶(3-phosphoglycerol dehydrogenase,GDH)、3-羟酰辅酶A脱氢酶(3-hydroxyacyl coenzyme A dehydrogenase,HOAD)、柠檬酸合成酶(citrate synthase,CS)和乳酸脱氢酶(lactate dehydrogenase,LDH)的活性,分析GAPDH/HOAD活性比值与地理纬度和海拔间的相关性。【结果】不同地理种群大豆食心虫老熟幼虫体内5种呼吸代谢关键酶的活性在不同温度下均有显著差异。其中,GAPDH/HOAD活性比值与种群所在地理纬度及海拔高度显著相关,18℃时大豆食心虫老熟幼虫体内GAPDH/HOAD活性比值与地理纬度及地理纬度叠加海拔转换纬度呈正相关,其回归曲线分别拟合二次多项式回归模型y=-0.1312+0.0313x-0.0003x^(2)和y=1.0905-0.0391x+0.0007x^(2);21℃时,大豆食心虫老熟幼虫体内GAPDH/HOAD活性比值与地理纬度及海拔叠加地理纬度呈负相关,其回归曲线分别拟合二次多项式回归模型y=-0.1350-0.0568x-0.0010x^(2)和y=0.8334-0.0002x-0.0002x^(2);24℃时,大豆食心虫老熟幼虫体内GAPDH/HOAD活性比值与地理纬度及地理纬度叠加海拔转换纬度也呈负相关,分别拟合二次多项式回归模型y=0.9268+0.0973x-0.0015x^(2)和y=1.3423-0.0215x+0.0006x^(2)。【结论】不同地理种群间在糖代谢、脂代谢、三羧酸循环代谢以及糖酵解水平上均存在差异,大豆食心虫老熟幼虫可通过调节呼吸代谢强度来适应环境温度变化,其呼吸代谢能源物质利用及其对环境温度的适应能力与种群所生存环境的地理纬度和海拔密切相关。
【Aim】To clarify the adaptation of different geographic populations of Leguminivora glycinivorella to the temperature changes by determining the activities of the key enzymes involved in respiratory metabolism in mature larvae of different geographic populations of this insect in response to temperature.【Methods】The activities of the key enzymes involved in respiratory metabolism,including 3-phosphoglyceraldehyde dehydrogenase(GAPDH),3-phosphoglycerol dehydrogenase(GDH),3-hydroxyacyl coenzyme A dehydrogenase(HOAD),citrate synthase(CS)and lactate dehydrogenase(LDH),in mature larvae of four geographic populations of L.glycinivorella from Du′an,Guangxi,Zunyi,Guizhou,Suzhou,Anhui and Changchun,Jilin at the temperatures of 18,21,24,27 and 30℃,respectively,by using biochemical methods.The correlations between the ratio of GAPDH activity to HOAD activity and the geographic latitude and altitude were analyzed.【Results】The activities of five key enzymes involved in respiratory metabolism in mature larvae of L.glycinivorella of different geographic populations were significantly different at different temperatures.Among them,the ratio of GAPDH activity to HOAD activity was significantly correlated with the geographic latitude and altitude where the populations were located,and the ratio of GAPDH activity to HOAD activity in L.glycinivorella mature larvae was positively correlated with the geographic latitude and the geographic latitude superimposed with altitude-converted latitude at 18℃,with their regression curves fitted with the quadratic polynomial regression models y=-0.1312+0.0313x-0.0003x^(2) and y=1.0905-0.0391x+0.0007x^(2),respectively.The ratio of GAPDH activity to HOAD activity in L.glycinivorella mature larvae was negatively correlated with geographic latitude and altitude-converted latitude superimposed with geographic latitude at 21℃,with their regression curves fitted the quadratic polynomial regression models y=-0.1350-0.0568x-0.0010x^(2) and y=0.8334-0.0002x-0.0002x^(2),respectively.The ratio of GAPDH activity to HOAD activity in L.glycinivorella mature larvae was also negatively correlated with geographic latitude and geographic latitude superimposed with altitude-converted latitude at 24℃,with their regression curves fitted the quadratic polynomial regression models y=0.9268+0.0973x-0.0015x^(2) and y=1.3423-0.0215x+0.0006x^(2),respectively.【Conclusion】Among different geographic populations,there are differences in carbohydrate metabolism,lipid metabolism,tricarboxylic acid cycle metabolism and the level of glycolysis.L.glycinivorella mature larvae can adapt to the changes in ambient temperature by regulating the intensity of respiratory metabolism,and the utilization of energy substances in their respiratory metabolism and their ability to adapt to ambient temperature are closely related to the geographic latitude and altitude of the environment in which they live.
作者
邢一唱
朱诗禹
李宏鹏
王小奇
崔娟
田径
毕锐
高宇
史树森
XING Yi-Chang;ZHU Shi-Yu;LI Hong-Peng;WANG Xiao-Qi;CUI Juan;TIAN Jing;BI Rui;GAO Yu;SHI Shu-Sen(Key Laboratory of Soybean Disease and Pest Control,Ministry of Agriculture and Rural Affairs,College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Changchun Greening Management Center,Changchun 130022,China;Tonghua Meteorological Bureau of Jilin,Tonghua 134000,China;Jilin Agriculture Science and Technology College,Jilin 132101,China)
出处
《昆虫学报》
北大核心
2025年第11期1510-1520,共11页
Acta Entomologica Sinica
基金
国家重点研发计划项目(2023YFD1401000)
财政部和农业农村部:国家现代农业产业技术体系(CARS-04)
吉林省科技厅重点研发项目(20240303023NC)。
关键词
大豆食心虫
温度
地理种群
呼吸代谢
酶活性
Leguminivora glycinivorella
temperature
geographic population
respiratory metabolism
enzyme activity
