摘要
目的 探讨血清着丝粒相关蛋白U(CENPU) mRNA、三基序蛋白27(TRIM27)联合经阴道剪切波弹性成像(SWE)对宫颈癌的早期诊断价值。方法 选取2022年1月至2023年1月河北生殖妇产医院收治的宫颈病变患者100例作为研究对象,所有患者均行经阴道SWE检测,将46例经组织活检确诊为宫颈癌患者作为恶性组,54例宫颈上皮内瘤变患者作为良性组,另选同期体检且无宫颈疾病的健康志愿者50例作为对照组。采用定量反转录聚合酶链反应(qRT-PCR)检测CENPU mRNA水平,采用酶联免疫吸附试验检测TRIM27水平。绘制受试者工作特征(ROC)曲线分析血清CENPU mRNA、TRIM27联合经阴道SWE诊断宫颈癌的价值。结果 与对照组比较,良性组、恶性组血清CENPU mRNA、TRIM27水平升高(P<0.05)。与良性组比较,恶性组血清CENPU mRNA、TRIM27水平升高(P<0.05)。恶性组平均弹性模量、最大弹性模量显著高于良性组和对照组(P<0.05)。血清CENPU mRNA、TRIM27、平均弹性模量、最大弹性模量诊断宫颈癌的曲线下面积(AUC)分别为0.824、0.845、0.860、0.863,4项联合诊断宫颈癌的AUC为0.975,优于各指标单独诊断(Z_(4项联合vs. CENPU mRNA)=2.712、Z_(4项联合vs.TRIM27)=2.736、Z_(4项联合vs.平均弹性模量)=2.765、Z_(4项联合vs.最大弹性模量)=2.778,均P<0.05)。结论 宫颈癌患者血清CENPU mRNA、TRIM27水平明显升高,血清CENPU mRNA、TRIM27联合经阴道SWE可提高对宫颈癌的早期诊断价值。
Objective To investigate the early diagnostic value of serum centromere protein U(CENPU)mRNA and tripartite motif-containing protein 27(TRIM27)combined with transvaginal shear wave elastography(SWE)in cervical cancer.Methods A total of 100 patients with cervical lesions who were admitted to Hebei Reproductive and Maternity Hospital from January 2022 to January 2023 were enrolled in this study.All patients underwent SWE.Based on histopathological biopsy results,46 patients with cervical cancer were assigned to the malignant group,and 54 patients with cervical intraepithelial neoplasia were assigned to the benign group.In addition,50 healthy volunteers without cervical disease who underwent physical examinations during the same period were selected as the control group.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect serum CENPU mRNA level,and enzyme-linked immunosorbent assay(ELISA)was used to measure TRIM27 level.Receiver operating characteristic(ROC)curve was plotted to evaluate the diagnostic value of serum CENPU mRNA and TRIM27 combined with transvaginal SWE for cervical cancer.Results Compared with the control group,serum CENPU mRNA and TRIM27 levels were significantly elevated in both the benign and malignant groups(P<0.05).Compared with the benign group,serum CENPU mRNA and TRIM27 levels were further increased in the malignant group(P<0.05).The mean and maximum elastic modulus obtained by SWE were significantly higher in the malignant group than those in the benign and control groups(P<0.05).The areas under the curve(AUC)for diagnosing cervical cancer using serum CENPU mRNA,TRIM27,mean elastic modulus and maximum elastic modulus were 0.824,0.845,0.860,and 0.863 respectively.The combined detection of all four parameters yielded an AUC of 0.975,which was superior to that of any single indicator(Z_(combined vs.CENPU mRNA)=2.712,Z_(combined vs.TRIM27)=2.736,Z combined vs.mean elastic modulus=2.765,Z_(combined vs.maximum elastic modulus)=2.778;all P<0.05).Conclusion Serum CENPU mRNA and TRIM27 levels are significantly elevated in patients with cervical cancer.The combination of serum CENPU mRNA and TRIM27 with transvaginal SWE improves the early diagnostic value for cervical cancer.
作者
赵健
魏红
张平平
崔浩浩
张剑茹
ZHAO Jian;WEI Hong;ZHANG Pingping;CUI Haohao;ZHANG Jianru(Department of Ultrasound,Hebei Reproductive and Maternity Hospital,Shijiazhuang,Hebei 050000,China;Department of Obstetrics,the Fourth People′s Hospital of Hengshui,Hengshui,Hebei 053000,China)
出处
《检验医学与临床》
2025年第24期3393-3397,共5页
Laboratory Medicine and Clinic
基金
河北省医学科学研究课题计划(20241190)。
关键词
着丝粒相关蛋白U
三基序蛋白27
经阴道剪切波弹性成像
宫颈癌
弹性模量
centromere protein U
tripartite motif-containing protein 27
transvaginal shear wave elastography
cervical cancer
elastic modulu
