摘要
目的 探讨不明原因复发性流产(URSA)患者血清微小RNA-371a-5p(miR-371a-5p)、信号转导及转录激活蛋白3(STAT3)水平及临床意义。方法 前瞻性选取2021年1月至2023年1月该院收治的再次妊娠的URSA患者154例(URSA组)和同期在该院产检的健康孕妇80例(对照组)作为研究对象,根据是否发生流产将URSA患者分为流产组和未流产组。采用实时荧光定量聚合酶链反应与酶联免疫吸附试验分别检测血清miR-371a-5p、STAT3水平。通过Pearson相关分析URSA患者血清miR-371a-5p水平与STAT3水平的相关性;采用多因素非条件Logistic回归分析URSA患者再次发生流产的影响因素;采用受试者工作特征(ROC)曲线分析血清miR-371a-5p、STAT3对URSA患者再次发生流产的预测效能。结果 与对照组比较,URSA组血清miR-371a-5p水平升高(P<0.05),STAT3水平降低(P<0.05)。URSA患者血清miR-371a-5p水平与STAT3水平呈负相关(r=-0.781,P<0.001)。154例URSA患者再次流产率为42.21%(65/154)。URSA患者再次发生流产的独立危险因素为年龄≥30岁、流产次数≥4次和miR-371a-5p≥1.57(P<0.05),独立保护因素为STAT3≥8.58 ng/mL(P<0.05)。血清miR-371a-5p、STAT3联合预测URSA患者再次发生流产的曲线下面积为0.883,大于血清miR-371a-5p、STAT3单独预测的0.789、0.796(均P<0.05)。结论 URSA患者血清miR-371a-5p水平升高和STAT3水平降低,二者均与URSA患者再次发生流产密切相关,血清miR-371a-5p、STAT3联合预测URSA患者再次发生流产的效能较高。
Objective To investigate the serum levels of microRNA-371a-5p(miR-371a-5p)and signal transducer and activator of transcription 3(STAT3)in patients with unexplained recurrent spontaneous abortion(URSA)and to explore their clinical significance.Methods A total of 154 pregnant patients with URSA who conceived again and were treated at the hospital from January 2021 to January 2023 were prospectively enrolled as the URSA group,and 80 healthy pregnant women who underwent prenatal examination at the hospital during the same period were served as the control group.The URSA patients were further divided into the abortion group and the non-abortion group according to the occurrence of subsequent abortion.Serum levels of miR-371a-5p level and STAT3 were detected using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay respectively.The correlation between serum miR-371a-5p and STAT3 level was analyzed using Pearson correlation analysis.Multivariate unconditional Logistic regression analysis was performed to identify risk factors associated with subsequent abortion in URSA patients.The predictive performance of serum miR-371a-5p and STAT3 for subsequent abortion was evaluated using receiver operating characteristic(ROC)curve.Results Compared with the control group,serum miR-371a-5p level was significantly increased in the URSA group(P<0.05),whereas STAT3 level was significantly decreased(P<0.05)in the URSA group.Serum miR-371a-5p level was negatively correlated with STAT3 level in patients with URSA(r=-0.781,P<0.001).Among the 154 URSA patients,the recurrent abortion rate was 42.21%(65/154).Independent risk factors for recurrent abortion in URSA patients were age≥30 years,number of previous abortions≥4 and miR-371a-5p≥1.57(P<0.05),while STAT3≥8.58 ng/mL served as an independent protective factor(P<0.05).The combined detection of serum miR-371a-5p and STAT3 for predicting subsequent abortion in URSA patients yielded an area under the ROC curve of 0.883,which was greater than that of miR-371a-5p(0.789)or STAT3(0.796)alone(both P<0.05).Conclusion Elevated serum miR-371a-5p level and decreased STAT3 level in patients with URSA are closely associated with the occurrence of subsequent abortion.The combined detection of serum miR-371a-5p and STAT3 demonstrates a high predictive value for subsequent abortion in URSA patients.
作者
亓云芳
鹿玲
刘坤
QI Yunfang;LU Ling;LIU Kun(Department of Obstetrics,Jinan Second Maternal and Child Health Care Hospital,Jinan,Shandong 271100,China;Department of Gynecology,Jinan Second Maternal and Child Health Care Hospital,Jinan,Shandong 271100,China)
出处
《检验医学与临床》
2025年第24期3340-3345,共6页
Laboratory Medicine and Clinic
基金
山东省医药卫生科技发展计划项目(2020WS063)。
