摘要
生物降解是褐煤生物转化和高值化利用的重要途径。以内蒙古蒙东地区褐煤为研究对象,通过富集培养、形态学观察及ITS序列分析,成功筛选并鉴定了具有高效褐煤降解能力的青霉菌PPLL2205。利用扫描电子显微镜和傅里叶红外光谱仪对PPLL2205降解后的褐煤残渣进行结构解析,探究了PPLL2205对褐煤的物理侵蚀和化学结构的改性。利用紫外-可见分光光度计和气相色谱质谱联用仪对PPLL2205降解后的褐煤溶出液进行成分分析,明确了生物降解过程中释放的多种有机化合物。胞外酶活性分析表明,PPLL2205主要通过分泌木质素过氧化物酶、氧化酶及漆酶等,有效促进褐煤的生物降解。本研究不仅丰富了微生物降解褐煤的机理认识,也为低阶煤的清洁高效利用开辟了新途径。
Biodegradation is an important pathway for the biological transformation and high-value utilization of lignite.This study focused on lignite from Mengdong area of Inner Mongolia and successfully screened and identified Penicillium pimiteouiense(PPLL2205)with high lignite degradation capability through enriched cultivation,morphological observation,and ITS sequence analysis.The structure of lignite residue was analyzed using scanning electron microscope and fourier transform infrared spectroscopy to explore the physical erosion and chemical modification of lignite by PPLL2205.The ultraviolet-visible spectrophotometer and gas chromatography-mass spectrometry were used to analyze the chemical components of the lignite-solubilizing products after degradation by PPLL2205,clarifying the various organic compounds released during the biodegradation process.The extracellular enzyme activity analysis showed that PPLL2205 mainly promotes the biodegradation of lignite by secreting lignin peroxidase,oxidase,and laccase,etc.This study not only enriched the understanding of the mechanisms of microbial degradation of lignite but also opened up new avenues for the clean and efficient utilization of low-rank coal.
作者
刘雨鹤
崔丽萍
田保华
梁丹丹
王双林
李佳勇
梁海霞
李欣欣
LIU Yuhe;CUI Liping;TIAN Baohua;LIANG Dandan;WANG Shuanglin;LI Jiayong;LIANG Haixia;LI Xinxin(School of Environment and Ecology,Taiyuan University of Technology,Jinzhong 030600,China;School of Biomedical Engineering,Taiyuan University of Technology,Jinzhong 030600,China;Shanxi Xinhua Chemical Defense Equipment Research Institute Co.,Ltd.,Taiyuan 030008,China)
出处
《应用化工》
北大核心
2025年第11期2975-2980,共6页
Applied Chemical Industry
基金
国家自然科学基金青年基金项目(31501124)
山西省基础研究计划项目(202203021221046,202203021212197)。
关键词
内蒙褐煤
青霉菌
生物降解
芳香环开环氧化
醚键断裂
Inner Mongolian lignite
Penicillium
biodegradation
aromatic ring-opening oxidation
ether linkage
