摘要
交联质谱(cross-linking mass spectrometry,XL-MS)技术已成为鉴定蛋白质-蛋白质相互作用(protein-protein interactions,PPIs)以及揭示活细胞、组织或细胞器中蛋白质结构的关键工具。相较于传统PPI及蛋白质结构研究方法,XLMS技术展现出显著的优越性。近年来,新型交联剂结构不断涌现,不仅扩大了交联反应的范围,还提供了更多的交联肽富集方式;与此同时,得益于计算机软件及人工智能的发展,交联数据搜索引擎和可视化软件也在不断发展,提高了交联肽鉴定的速度及准确度。交联剂以及交联肽搜索引擎的发展促进了XL-MS技术在细胞器乃至活细胞内PPI研究以及蛋在白质结构解析中的应用。随着质谱技术及分析软件的不断进步,在未来XL-MS将在蛋白质相互作用研究和蛋白质结构解析领域扮演更加重要的角色。
Cross-linking mass spectrometry(XL-MS)technology has become a key tool for identifying proteinprotein interactions(PPIs)and revealing the protein structures in living cells,tissues,or organelles.The XL-MS technology has demonstrated remarkable superiority compared with conventional methods for studying PPIs and protein structures.In the past few years,new cross-linker structures have been arising continuously.They have not only broadened the range of cross-linking reactions but also provided additional methods for the enrichment of cross-linking peptides.Moreover,owing to the advancement of computer software and artificial intelligence,cross-linking data search engines and visualization software have also been enhancing the speed and accuracy of cross-linking peptide identification.The evolution of cross-linkers and cross-linking peptide search engines has promoted the application of the XL-MS technology in the research on protein-protein interactions(PPIs)within organelles and even living cells and in the analysis of protein structures.
作者
王茂琳
刘妍
严方
孙桂斌
WANG Maolin;LIU Yan;YAN Fang;SUN Guibin(Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009;Department of Pharmacy,Harbin Institute of Technology Hospital,Harbin 150000;Nanjing Xiluosi Pharmaceutical Co.,Ltd.,Nanjing 210038,China)
出处
《中国药科大学学报》
北大核心
2025年第6期793-802,共10页
Journal of China Pharmaceutical University
基金
国家自然科学基金项目(No.82173956)。
关键词
交联质谱
蛋白质相互作用
蛋白质结构
蛋白质组学
cross-linking mass spectrometry
protein-protein interactions
protein structure
proteomics
