摘要
本文旨在优化超声波辅助低共熔溶剂提取拐枣果梗(HAP)总多酚的工艺并分析其抗氧化活性。通过低共熔溶剂含水量、料液比、超声波辅助提取时间和温度等因素结合正交试验设计优化HAP总多酚的提取工艺。结果显示,最佳提取条件如下:氯化胆碱与苹果酸摩尔比1:1配制而成的低共熔溶剂,含水量为30%,料液比为1:30g/mL,超声时间为40 min,超声温度为50℃。在此条件下,HAP总多酚的提取质量分数为28.37 mgg。抗氧化分析结果表明,HAP总多酚对DPPH和ABTS自由基的IC_(50)分别为0.062和0.198 mg/mL,虽弱于维生素C的抗氧化能力,但HAP总多酚呈现显著的自由基清除活性和还原力。超声波辅助低共熔溶剂法是一种高效提取HAP总多酚的方法且提取物具有显著的抗氧化活性,在食品保鲜领域具有潜在的应用价值。
The aim was to optimize the process of extracting total polyphenols from Hovenia acerba with ultrasonic-assisted deep eutectic solvents(DESs)and analyze its antioxidant activity.The extraction process of total polyphenols from Hovenia acerba was optimized through orthogonal experimental design,considering factors such as the water content of DESs,solid-to-liquid ratio,ultrasonicassisted extraction time,and temperature.The results showed that the optimal extraction conditions were as follows:the molar ratio of choline chloride to malic acid was 1:1,the water content was 30%,the ratio of naterial to liquid was 1:30 g/mL,the ultrasonic time was 40 min,and the ultrasonic temperature was 50C.Under these conditions,the extraction yield of total polyphenols from Hovenia acerba was 28.37 mg/g.Antioxidant analysis results indicated that the IC_(50) values of total polyphenols from Hovenia acerba on DPPH radicals and ABTS radicals were 0.062 and 0.198 mg/mL,respectively,which were weaker than the antioxidant capacity of vitamin C.However,taken together,the total polyphenols from Hovenia acerba still exhibited significant radical scavenging activity and reducing power.The ultrasonic-assisted DES method is an efficient approach for extracting total polyphenols from Hovenia acerba,and the extract has significant antioxidant activity,suggesting potential application value in the field of food preservation.
作者
侯贺丽
任双
石雨萌
杨玲玲
耿书宝
朱静
HOU He-li;REN Shuang;SHI Yu-meng;YANG Ling-ling;GENG Shu-bao;ZHU Jing(School of Food Sciences and Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China)
出处
《化学研究与应用》
北大核心
2025年第12期3691-3700,共10页
Chemical Research and Application
基金
河南省科技攻关项目(242102110090,242102110178)资助
河南省自然科学基金面上项目(242300420173)资助
河南省高等学校重点科研项目(23A550015)资助
河南省本科高校2023年度产教融合项目(47)资助
信阳农林学院科技创新团队项目(XNKJTD-001)资助。
关键词
拐枣
超声波辅助提取
低共熔溶剂
总多酚
抗氧化活性
Hovenia acerba
ultrasonic-assisted extraction
deep eutectic solvent
total polyphenols
antioxidant activity
