摘要
以无花果叶为原料,通过用双水相体系(Aqueous two-phase system,ATPS)结合超声技术对无花果叶多糖的提取工艺进行优化以提高其多糖得率,并对其体外抗氧化活性分析。首先考察了不同ATPS的分配行为得出最佳ATPS溶剂,再采用单因素对料液比、超声处理时间、温度和功率进行了考察,在此基础上用响应面法对无花果叶多糖的提取工艺优化,并分析其体外抗氧化活性和降血脂能力。最佳ATPS溶剂为质量分数12%聚乙二醇6000(PEG6000)和16%(NH_(4))_(2)SO_(4),最佳提取工艺条件为料液比1:22 g·mL^(-1),超声提取功率360W,超声提取时间25 min,超声波提取温度50℃。在此条件下,多糖得率最高可达13.36±0.08%,与预测值接近。无花果叶多糖抗氧化能力及胰脂肪酶抑制能力随着浓度提高而增强,其清除DPPH·和·OH的IC_(50)值分别为0.33和0.32 mg·L^(-1),其对胰脂肪酶抑制率的IC_(50)值分别为0.94 mg·mL^(-1),说明具有较好的抗氧化性能和体外降血脂活性。双水相法提取工艺稳定、可行,可用于无花果叶多糖的提取,为进一步纯化植物多糖奠定了基础,也为植物多糖的提取提供了一种有价值的替代方法。
In this paper the extraction process of polysaccharides from fig leaves using a two-phase aqueous system(ATPS)combined with ultrasonic technology was optimized,the yield of polysaccharides was improved,and their antioxidant activity was analyzed.Firstly,the optimal ATPS solvent was obtained by investigating the distribution behavior of different ATPSs.Then,the solidliquid ratio,ultrasonic treatment time,temperature and power were investigated through single-factor experiments.On this basis,the extraction process of polysaccharides from fig leaves was optimized using response surface methodology.The removal ability of DPPH·and·OH was studied with Vc as a control.The optimal ATPS solvent was 12%polyethylene glycol 6000(PEG6000)and 16%(NH_(4))_(2)SO_(4) by mass fraction.The optimal extraction process conditions were a material-to-liquid ratio of 1:22 g·mL^(-1)an ultrasonic extraction power of 360 W,an ultrasonic extraction time of 25 min,and ultrasonic extraction temperature of 50℃.Under these optimal conditions,the highest polysaccharide yield could reach 13.36±0.08%,which was close to the predicted value.The antioxidant capacity and pancreatic lipase inhibitory activity of fig leaf polysaccharides increasd with the increasing concentration.The IC_(50)values for scavenging DPPH·and·OH were 0.33 mg·L^(-1)and 0.32 mg·L^(-1)respectively;and the IC_(50)value for inhibiting pancreatic lipase was 0.94 mg·mL^(-1),indicating that it had good antioxidant properties and in vitro lipid-lowering activity.The two-phase aqueous extraction process was stable and feasible,which could be used for extracting fig leaf polysaccharides,while laying a foundation for the further purification of plant polysaccharides and providing a valuable alternative method for their extraction.
作者
孙伟
刘林东
叶润
赵文凤
蔡静
赵丽平
SUN Wei;LIU Lin-dong;YE Run;ZHAO Wen-feng;CAI Jing;ZHAO Li-ping(School of Pharmacy,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Public Education Department,Xinyang Aviation Vocational College,Xinyang 464000,China)
出处
《化学研究与应用》
北大核心
2025年第12期3605-3614,共10页
Chemical Research and Application
基金
河南省重点研发专项项目(241111311400)资助
河南省科技攻关项目(222102110247,222102110449)资助
信阳农林学院大别山道地中药资源与系列产品开发研究科技创新团队项目(XNKJTD-009)资助。
关键词
无花果叶
双水相体系
多糖
响应面设计
抗氧化
胰脂肪酶抑制率
fig leaves
two-phase aqueous system
polysaccharides
response surface design
antioxidant activity
inhibition rate of pancreatic lipase
