摘要
细菌生物被膜是由细菌分泌的胞外聚合物包裹而形成的菌体聚集膜状物。其对抗生素、紫外线、化学杀菌剂以及宿主免疫均具备高度耐受性。大约80%的顽固和难治性感染与生物被膜的形成紧密相关,这已然成为临床感染防控工作中的重大挑战。噬菌体作为能够特异性感染细菌的病毒,具有生产周期短、成本低、环境友好等特点,在裂解临床致病菌形成的生物被膜中展现出巨大的应用潜力。本文基于国内外的研究报道,从噬菌体鸡尾酒疗法、噬菌体-抗生素联合疗法、噬菌体基因编码产物(如解聚酶和内溶素)及基因工程噬菌体等方面对噬菌体裂解临床致病菌生物被膜的研究进行了综述,旨在为噬菌体裂解生物被膜的临床应用以及新型抗生物被膜制剂的开发提供理论依据。
Bacterial biofilms are membrane-like aggregates formed by bacterial communities encased within self-secreted extracellular polymeric substances.These structures exhibit high tolerance to antibiotics,ultraviolet radiation,chemical disinfectants,and host immune responses.Approximately 80%of persistent and refractory infections are closely associated with biofilm formation,which has become a major challenge in clinical infection prevention and control.Bacteriophages(phages),viruses that specifically infect bacteria,offer significant application potential in lysing biofilms formed by clinical pathogenic bacteria due to their short replication cycles,low cost,and environmental friendliness.Based on domestic and international research reports,this article reviews advances in phage-mediated lysis of clinical pathogenic bacterial biofilms,focusing on phage cocktail therapy,phage-antibiotic combination therapy,phage-encoded products(such as depolymerases and endolysins),and genetically engineered phages.The aim is to provide a theoretical foundation for the clinical application of phage-based biofilm lysis and the development of novel anti-biofilm agents.
作者
孙立婷
余广超
SUN Li-ting;YU Guang-chao(Clinical Laboratory Center,the First Affiliated Hospital of Jinan University,Guangzhou 510630,Guangdong,CHINA)
出处
《海南医学》
2025年第24期3641-3648,共8页
Hainan Medical Journal
