摘要
目的了解一起产妇和新生儿感染单增李斯特菌的流行病学特征,并对分离株进行病原学和全基因组溯源分析,为李斯特菌病的防治提供参考依据。方法收集病例信息,开展现场流行病学调查,采集相关生物、环境、食品样本进行细菌分离鉴定,对分离株进行药物敏感性试验和全基因组测序,对耐药基因、毒力基因作出分析,进行多位点序列分型(MLST)、核心基因组多位点序列分型(cgMLST)和核心基因组单核苷酸多态性分析(cgSNP)。结果产妇、患儿及环境、食品样本共分离单增李斯特菌6株,其中ST8型5株,ST224型1株;cgMLST及cgSNP分析结果显示孕妇、患儿生物样本及奶瓶奶嘴样本分离株全基因组序列高度相似,且与陕西省历史分离的ST8型菌株聚集成簇,但cgSNP有较大差异;分离株对青霉素等抗生素无耐药现象;分离株均携带inlA等毒力因子。结论本起新生儿感染单增李斯特菌案例系母婴垂直感染,感染源可能为该产妇孕晚期所食熟食品;ST8型单增李斯特菌在陕西省内长期流行,应加强单增李斯特菌的长期监测与防治。
In this study,the epidemiological characteristics of a maternal and neonatal infection with Listeria monocytogenes were studied,and the etiology and genome-wide traceability of isolates were analyzed,to provide a reference for the prevention and control of listeriosis.We collected case information;conducted on-site epidemiological investigations;collected relevant biological,environmental,and food samples for bacterial isolation and identification;performed drug sensitivity tests and whole-genome sequencing on the isolated strains;analyzed the resistance genes and virulence genes;and carried out multilocus sequence typing(MLST),core genome multilocus sequence typing(cgMLST),and core genome single nucleotide polymorphism(cgSNP)analyses.Six strains of L.monocytogenes were isolated from maternal and pediatric patients,and from environmental and food samples.Five strains were of ST8 type,and one strain was of ST224 type.The results of cgMLST and cgSNP analysis indicated that the whole genome sequences of the isolates from the biological samples of pregnant patients,pediatric patients,and baby bottle nipple samples were completely identical,and clustered with the historical ST8-type strains isolated in Shaanxi Province.However,significant differences in cgSNP were observed.The isolates showed no resistance to antibiotics such as penicillin.All isolates carried virulence factors such as inlA.This case of neonatal infection with L.monocytogenes was a vertical infection from mother to child.The source of infection might have been cooked food that the parturient consumed during late pregnancy.ST8 type L.monocytogenes has been endemic in Shaanxi Province for a long time period.Long-term L.monocytogenes monitoring and prevention and control efforts should be strengthened.
作者
梁爽
王增国
马国柱
刘长宏
李慎
史伟
刘东立
LIANG Shuang;WANG Zengguo;MA Guozhu;LIU Changhong;LI Shen;SHI Wei;LIU Dongli(Shaanxi Center for Disease Control and Prevention,Xi'an 710054,China;Xi'an Children's Hospital,Xi'an 710000,China)
出处
《中国人兽共患病学报》
北大核心
2025年第11期1185-1191,共7页
Chinese Journal of Zoonoses
基金
陕西省2023年重点研发计划(No.2023-YBSF-633)。
