摘要
目的分析天津市羊种布鲁氏菌临床药物敏感和毒力特征,了解本地羊种布鲁氏菌耐药、致病风险,提供临床和公共卫生学价值。方法选取2020—2022年天津市国家级布病监测哨点医疗机构的布病患者血样中分离的羊种布鲁氏菌分离株进行药敏试验、全基因组测序,并对数据进行耐药表型、耐药基因及毒力基因分析。结果12株羊种布鲁氏菌中有7株菌对氨苄西林中介,3株菌对氨苄西林耐受;所有菌株对红霉素、复方新诺明的敏感性大大降低,有3株菌的复方新诺明MIC值已处于药物敏感的临界值。所有菌株均预测出6个相同的耐药基因,包括1个肽类抗性因子,5个外排泵转运相关基因。全部菌株毒力基因分布没有差异,共预测出65个毒力基因。结论天津地区首次发现有对氨苄西林耐药的人源菌株,同时检出红霉素及复方新诺明呈现高MIC值的菌株;所有菌株主要携带T4SS及其效应子、免疫调控类毒力基因;提示需高度重视此类耐药菌株的临床传播风险,并加强相关病原学及耐药性监测。
This study was to analyze the clinical drug sensitivity and virulence characteristics of the bacterial genus Brucella in Tianjin,and to understand the drug resistance and pathogenic risk of Brucella in local sheep,to provide clinical and public health value.Brucella melitensis isolates from blood samples from patients with brucellosis at national brucellosis surveillance sentinel medical institutions in Tianjin in 2020—2022 were selected for drug susceptibility testing and whole genome sequencing.The data were analyzed for drug resistance phenotype,drug resistance genes,and virulence genes.Of the 12 B.melitensis strains,seven were ampicillin intermediates,and three were ampicillin tolerant.The sensitivity of all strains to erythromycin and cotrimoxazole was markedly diminished,and the MIC value of cotrimoxazole of three strains was at the boundary value of drug sensitivity.All strains predicted six identical resistance genes,one peptide resistance factor,and five effluent-pump transport-related genes.No difference in virulence gene distribution was observed among strains,and a total of 65 virulence genes were predicted.In summary,the first human strain resistant to ampicillin was discovered in Tianjin,and strains with high MIC values for erythromycin and compound sulfamethoxazole were detected.All strains carried primarily T4SS and its effectors,as well as virulence genes associated with immunoregulation.This discovery provides important evidence to support precise clinical anti-infection treatment.Moreover,it suggests that the clinical transmission risk of such drug-resistant strains is critical,and related etiological and drug resistance monitoring measures should be strengthened.
作者
郑宝璐
李闻
郭丽茹
吕莉琨
谭昭麟
谢彤
张维
李晓燕
ZHENG Baolu;LI Wen;GUO Liru;LYU Likun;TAN Zhaolin;XIE Tong;ZHANG Wei;LI Xiaoyan(Tianjin Key Laboratory of Pathogenic Microorganisms for Infectious Diseases,Institute of Microbiology,Tianjin Center for Disease Control and Prevention,Tianjin 300011,China;Key Laboratory of Prevention and Control of Major Diseases in the Population,Ministry of Education,School of Public Health,Tianjin Medical University,Tianjin 300203,China)
出处
《中国人兽共患病学报》
北大核心
2025年第11期1159-1165,共7页
Chinese Journal of Zoonoses
基金
天津市卫生健康科技项目(No.TJWJ2022ZD010)。
关键词
羊种布鲁氏菌
耐药
氨苄西林
红霉素
复方新诺明
Brucella melitensis
drug resistance
ampicillin
erythromycin
cotrimoxazole
