摘要
目的:准确评估区域淋巴结转移状态对于口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)诊治至关重要,本研究拟利用碘基增强Micro-CT成像技术辅助病理切片三维评估OSCC区域淋巴结状态。方法:收集75枚OSCC患者离体淋巴结样本,经3%Lugol’s iodine染色12 h后行Micro-CT扫描。随后将样本行常规苏木精-伊红(hematoxylin-eosin,HE)染色切片,制作数字化病理切片。随后对比Micro-CT图像与病理图像对淋巴结转移状态的评估情况。结果:碘基增强Micro-CT成像可以清晰勾勒淋巴结的转移灶,以二维病理切片为金标准,其特异度为90.4%,灵敏度为91.3%,准确度为90.7%。结论:碘基增强Micro-CT成像可以三维准确评估OSCC的离体淋巴结转移状态,有效降低单纯二维病理切片对微小淋巴结转移的漏诊,为OSCC术后辅助治疗的制定提供更准确的依据。
Objective:To evaluate the status of regional lymph nodes in oral squamous cell carcinoma(OSCC)with Lugol's iodine enhanced micro-computed tomography(Micro-CT)imaging.Methods:Seventy-five isolated lymph node samples were collected from OSCC patients,and were stained with 3%Lugol's iodine for 12 hours before Micro-CT scan.The samples were sliced by conventional hematoxylin-eosin(H&E)staining,and digital pathological sections were made.The evaluation of lymph node metastasis status by Micro-CT image and pathological image was compared.Results:Lugol's iodine enhanced Micro-CT imaging could delineate lymph node metastasis clearly.2D pathological sections were used as gold standard,with specificity of 90.4%,sensitivity of 91.3%,and accuracy of 90.7%.Conclusion:Lugol's iodine enhanced Micro-CT imaging can accurately evaluate the status of isolated lymph node metastasis in OSCC in three dimensions,effectively reduce the missed diagnosis of microlymph node metastasis in simple two-dimensional pathological sections,and provide a more accurate basis for the formulation of postoperative adjuvant treatment for OSCC.
作者
顾洲宇
贾辉
王育新
夏成万
GU Zhouyu;JIA Hui;WANG Yuxin;XIA Chengwan(Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China)
出处
《口腔医学研究》
北大核心
2025年第12期1065-1069,共5页
Journal of Oral Science Research
基金
国自然青年科学基金(编号:82403466)
南京市卫生科技发展项目(编号:YKK22177)
南京市口腔医院高水平医院建设科研项目(编号:0224c028)。
