摘要
本研究旨在探究贵州烟田杂草香附子(Cyperus rotundus)根系分泌物对烟草青枯病病原菌青枯雷尔氏菌(Ralstonia solanacearum)AS-1和生防菌贝莱斯芽孢杆菌(Bacillus velezensis)JXF-16的影响。采用非靶向代谢组学(LC-MS)技术对香附子根系分泌物的化学组成进行鉴定,并探究根系分泌物中部分活性物质对上述2种细菌的影响。使用比浊法测定根系分泌物活性物质对青枯菌和贝莱斯芽孢杆菌生长量的影响;采用结晶紫染色法测定其对细菌生物膜形成量的影响;利用半固体平板培养法评估其对青枯菌和贝莱斯芽孢杆菌运动活性的影响;最后通过盆栽防治试验评价外源活性物质对烟草青枯病的防治效果。结果表明:通过LC-MS分析共鉴定出21类化合物,共计3176种活性物质,其中肉豆蔻酸、4-乙基辛酸、川芎嗪和甜菜碱4种物质在浓度为50μmol/L时均显著促进青枯菌生长与生物膜形成,并促进其运动活性,但高浓度下4种物质均对其生长和生物膜形成呈抑制作用;50μmol/L川芎嗪和100μmol/L甜菜碱可有效促进贝莱斯芽孢杆菌生长和生物膜形成,并增强其运动活性;月桂酸和2-十二烷基苯磺酸浓度高于50μmol/L时均对青枯菌和贝莱斯芽孢杆菌的生长、生物膜形成及运动活性产生显著抑制作用(P<0.05)。盆栽防治试验表明,150、200μmol/L月桂酸和2-十二烷基苯磺酸对青枯病均具有较好防治效果,且相对防效均高于化学农药和生防菌处理,其中200μmol/L 2-十二烷基苯磺酸的相对防效高达80.28%。该研究结果不仅为阐释香附子根系分泌物在烟田生态系统中的作用机制提供重要理论依据,也为研发基于活性物质的烟草青枯病新型防治措施奠定基础。
This study aimed to investigate the effects of the root exudates from Cyperus rotundus,a common weed in Guizhou tabacco fields,on the tobacco bacterial wilt pathogen Ralstonia solanacearum strain AS-1 and the biocontrol agent Bacillus velezensis strain JXF-16.Non-targeted metabolomics(LC-MS)was employed to identify the chemical composition of C.rotundus root exudates and to explore the effects of specific bioactive constituents on the two bacterial strains.Turbidimetric assays were used to measure the impact of the active compounds on bacterial growth,crystal violet staining was applied to quantify biofilm formation,and semi-solid plate culture assays were conducted to assess bacterial motility.Additionally,pot experiments were performed to evaluate the efficacy of the exogenous active compounds in controlling bacterial wilt.Results revealed that a total of 21 types of chemical compounds and 3176 active substances were detected by LC-MS.At the concentration of 50μmol/L,myristic acid,4-ethyloctanoic acid,ligustrazine and betaine significantly promoted the growth,biofilm formation and motility of R.solanacearum.However,at higher concentrations,the compounds exhibited inhibitory effects on bacterial growth and biofilm formation.Tetramethylpyrazine(50μmol/L)and betaine(100μmol/L)significantly enhanced the growth,biofilm formation and motility of B.velezensis.Conversely,lauric acid and 2-dodecylbenzenesulfonic acid at concentrations above 50μmol/L significantly inhibited the growth,biofilm formation,and motility of both R.solanacearum and B.velezensis.Pot experiments demonstrated that lauric acid and 2-dodecylbenzenesulfonic acid at 150μmol/L and 200μmol/L effectively controlled bacterial wilt,with the relative control efficacies surpassing those of chemical pesticides and biocontrol agents.Notably,2-dodecylbenzenesulfonic acid at 200μmol/L achieved a control efficacy of 80.28%.The results would provide important theoretical insights into the role of C.rotundus root exudates within the tobacco field ecosystem and lay an applied foundation for developing novel bioactive compound-based strategies to control tobacco bacterial wilt.
作者
吴洪梅
吴浩锋
丁海霞
陆铮铮
彭丽娟
WU Hongmei;WU Haofeng;DING Haixia;LU Zhengzheng;PENG Lijuan(College of Tobacco Science,Guizhou University,Guiyang,Guizhou 550025,China;College of Agriculture,Guizhou University,Guiyang,Guizhou 550025,China;Zunyi Normal University,Zunyi,Guizhou 563002,China;Guizhou Provincial Key Laboratory of Tobacco Quality and Efficiency Improvement,Guiyang,Guizhou 550025,China)
出处
《热带作物学报》
北大核心
2025年第12期3009-3020,共12页
Chinese Journal of Tropical Crops
基金
贵州省自然科学基金重点项目(黔科合基础-ZK[2023]重点009)
合肥市科技计划项目(遵市科合HZ字[2025]171号)
贵州省烟草提质增效全省重点实验室项目(黔科合平台ZSYS[2025]028)。
关键词
香附子
青枯菌
根系分泌物
烟草
生长量
生物膜
运动活性
Cyperus rotundus
Ralstonia solanacearum
root exudates
tabacco
growth
biofilm
motility activity
